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PubMed:19233913 JSONTXT 9 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-185 Sentence denotes mRNA expression and BRAF mutation in circulating melanoma cells isolated from peripheral blood with high molecular weight melanoma-associated antigen-specific monoclonal antibody beads.
T1 0-185 Sentence denotes mRNA expression and BRAF mutation in circulating melanoma cells isolated from peripheral blood with high molecular weight melanoma-associated antigen-specific monoclonal antibody beads.
TextSentencer_T2 186-197 Sentence denotes BACKGROUND:
T2 186-197 Sentence denotes BACKGROUND:
TextSentencer_T3 198-395 Sentence denotes The detection of circulating tumor cells (CTCs) in the peripheral blood of melanoma patients by quantitative real-time reverse-transcription PCR (qRT-PCR) analysis correlates with a poor prognosis.
T3 198-395 Sentence denotes The detection of circulating tumor cells (CTCs) in the peripheral blood of melanoma patients by quantitative real-time reverse-transcription PCR (qRT-PCR) analysis correlates with a poor prognosis.
TextSentencer_T4 396-566 Sentence denotes The assessment of CTCs from blood has been difficult because of lack of a good monoclonal antibody (mAb) directed against surface cell antigens to capture melanoma cells.
T4 396-566 Sentence denotes The assessment of CTCs from blood has been difficult because of lack of a good monoclonal antibody (mAb) directed against surface cell antigens to capture melanoma cells.
TextSentencer_T5 567-575 Sentence denotes METHODS:
T5 567-575 Sentence denotes METHODS:
TextSentencer_T6 576-697 Sentence denotes Blood was collected prospectively from 57 melanoma patients (43 test and 14 test-development cases) and 5 healthy donors.
T6 576-697 Sentence denotes Blood was collected prospectively from 57 melanoma patients (43 test and 14 test-development cases) and 5 healthy donors.
TextSentencer_T7 698-870 Sentence denotes High molecular weight melanoma-associated antigen (HMW-MAA)-specific mAbs bound to immunomagnetic beads were used to isolate CTCs. mRNA and/or DNA were extracted from CTCs.
T7 698-870 Sentence denotes High molecular weight melanoma-associated antigen (HMW-MAA)-specific mAbs bound to immunomagnetic beads were used to isolate CTCs. mRNA and/or DNA were extracted from CTCs.
TextSentencer_T8 871-1114 Sentence denotes Testing for the expression of a melanoma-associated gene panel (MLANA, MAGEA3, and MITF) with qRT-PCR and for the presence of BRAFmt (a BRAF gene variant encoding the V600E mutant protein) verified the beads-isolated CTCs to be melanoma cells.
T8 871-1114 Sentence denotes Testing for the expression of a melanoma-associated gene panel (MLANA, MAGEA3, and MITF) with qRT-PCR and for the presence of BRAFmt (a BRAF gene variant encoding the V600E mutant protein) verified the beads-isolated CTCs to be melanoma cells.
TextSentencer_T9 1115-1186 Sentence denotes A peptide nucleic acid-clamping PCR assay was used for BRAFmt analysis.
T9 1115-1186 Sentence denotes A peptide nucleic acid-clamping PCR assay was used for BRAFmt analysis.
TextSentencer_T10 1187-1195 Sentence denotes RESULTS:
T10 1187-1195 Sentence denotes RESULTS:
TextSentencer_T11 1196-1488 Sentence denotes Spiking of peripheral blood cells (PBCs) with melanoma cells showed that the beads-based detection assay can detect approximately 1 melanoma cell in 5 x 10(6) PBCs. qRT-PCR analysis detected MLANA, MAGEA3, and MITF expression in 19 (44%), 29 (67%), and 19 (44%) of the patients, respectively.
T11 1196-1488 Sentence denotes Spiking of peripheral blood cells (PBCs) with melanoma cells showed that the beads-based detection assay can detect approximately 1 melanoma cell in 5 x 10(6) PBCs. qRT-PCR analysis detected MLANA, MAGEA3, and MITF expression in 19 (44%), 29 (67%), and 19 (44%) of the patients, respectively.
TextSentencer_T12 1489-1578 Sentence denotes At least one biomarker of the panel was positive in 40 (93%) of the 43 melanoma patients.
T12 1489-1578 Sentence denotes At least one biomarker of the panel was positive in 40 (93%) of the 43 melanoma patients.
TextSentencer_T13 1579-1657 Sentence denotes BRAFmt was detected in 17 (81%) of the 21 assessed stage IV melanoma patients.
T13 1579-1657 Sentence denotes BRAFmt was detected in 17 (81%) of the 21 assessed stage IV melanoma patients.
TextSentencer_T14 1658-1669 Sentence denotes CONCLUSION:
T14 1658-1669 Sentence denotes CONCLUSION:
TextSentencer_T15 1670-1848 Sentence denotes The assay of bead capture coupled with the PCR has utility for assessing CTCs in melanoma patients, which can then be characterized for both genomic and transcriptome expression.
T15 1670-1848 Sentence denotes The assay of bead capture coupled with the PCR has utility for assessing CTCs in melanoma patients, which can then be characterized for both genomic and transcriptome expression.