Id |
Subject |
Object |
Predicate |
Lexical cue |
TextSentencer_T1 |
0-149 |
Sentence |
denotes |
Adhesion forces in the self-recognition of oligosaccharide epitopes of the proteoglycan aggregation factor of the marine sponge Microciona prolifera. |
T1 |
0-149 |
Sentence |
denotes |
Adhesion forces in the self-recognition of oligosaccharide epitopes of the proteoglycan aggregation factor of the marine sponge Microciona prolifera. |
TextSentencer_T2 |
150-285 |
Sentence |
denotes |
Cell aggregation in the marine sponge Microciona prolifera is mediated by a multimillion molecular-mass aggregation factor, termed MAF. |
T2 |
150-285 |
Sentence |
denotes |
Cell aggregation in the marine sponge Microciona prolifera is mediated by a multimillion molecular-mass aggregation factor, termed MAF. |
TextSentencer_T3 |
286-505 |
Sentence |
denotes |
Earlier investigations revealed that the cell aggregation activity of MAF depends on two functional domains: (i) a Ca(2+)-independent cell-binding domain and (ii) a Ca(2+)-dependent proteoglycan self-interaction domain. |
T3 |
286-505 |
Sentence |
denotes |
Earlier investigations revealed that the cell aggregation activity of MAF depends on two functional domains: (i) a Ca(2+)-independent cell-binding domain and (ii) a Ca(2+)-dependent proteoglycan self-interaction domain. |
TextSentencer_T4 |
506-784 |
Sentence |
denotes |
Structural analysis of involved carbohydrate fragments of the proteoglycan in the self-association established a sulfated disaccharide beta-D: -GlcpNAc3S-(1-->3)-alpha-L: -Fucp and a pyruvated trisaccharide beta-D: -Galp4,6(R)Pyr-(1-->4)-beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp. |
T4 |
506-784 |
Sentence |
denotes |
Structural analysis of involved carbohydrate fragments of the proteoglycan in the self-association established a sulfated disaccharide beta-D: -GlcpNAc3S-(1-->3)-alpha-L: -Fucp and a pyruvated trisaccharide beta-D: -Galp4,6(R)Pyr-(1-->4)-beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp. |
TextSentencer_T5 |
785-997 |
Sentence |
denotes |
Recent UV, SPR, and TEM studies, using BSA conjugates and gold nanoparticles of the synthetic sulfated disaccharide, clearly demonstrated self-recognition on the disaccharide level in the presence of Ca(2+)-ions. |
T5 |
785-997 |
Sentence |
denotes |
Recent UV, SPR, and TEM studies, using BSA conjugates and gold nanoparticles of the synthetic sulfated disaccharide, clearly demonstrated self-recognition on the disaccharide level in the presence of Ca(2+)-ions. |
TextSentencer_T6 |
998-1168 |
Sentence |
denotes |
To determine binding forces of the carbohydrate-carbohydrate interactions for both synthetic MAF oligosaccharides, atomic force microscopy (AFM) studies were carried out. |
T6 |
998-1168 |
Sentence |
denotes |
To determine binding forces of the carbohydrate-carbohydrate interactions for both synthetic MAF oligosaccharides, atomic force microscopy (AFM) studies were carried out. |
TextSentencer_T7 |
1169-1471 |
Sentence |
denotes |
It turned out that, in the presence of Ca(2+)-ions, the force required to separate the tip and sample coated with a self-assembling monolayer of thiol-spacer-containing beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp-(1-->O)(CH(2))(3)S(CH(2))(6)S- was found to be quantized in integer multiples of 30 +/- 6 pN. |
T7 |
1169-1471 |
Sentence |
denotes |
It turned out that, in the presence of Ca(2+)-ions, the force required to separate the tip and sample coated with a self-assembling monolayer of thiol-spacer-containing beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp-(1-->O)(CH(2))(3)S(CH(2))(6)S- was found to be quantized in integer multiples of 30 +/- 6 pN. |
TextSentencer_T8 |
1472-1553 |
Sentence |
denotes |
No binding was observed between the two monolayers in the absence of Ca(2+)-ions. |
T8 |
1472-1553 |
Sentence |
denotes |
No binding was observed between the two monolayers in the absence of Ca(2+)-ions. |
TextSentencer_T9 |
1554-1610 |
Sentence |
denotes |
Cd(2+)-ions could partially induce the self-interaction. |
T9 |
1554-1610 |
Sentence |
denotes |
Cd(2+)-ions could partially induce the self-interaction. |
TextSentencer_T10 |
1611-1833 |
Sentence |
denotes |
In contrast, similar AFM experiments with thiol-spacer-containing beta-D: -Galp4,6(R)Pyr-(1-->4)-beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp-(1-->O)(CH(2))(3)S(CH(2))(6)S- did not show a binding in the presence of Ca(2+)-ions. |
T10 |
1611-1833 |
Sentence |
denotes |
In contrast, similar AFM experiments with thiol-spacer-containing beta-D: -Galp4,6(R)Pyr-(1-->4)-beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp-(1-->O)(CH(2))(3)S(CH(2))(6)S- did not show a binding in the presence of Ca(2+)-ions. |
TextSentencer_T11 |
1834-1983 |
Sentence |
denotes |
Also TEM experiments of gold nanoparticles coated with the pyruvated trisaccharide could not make visible aggregation in the presence of Ca(2+)-ions. |
T11 |
1834-1983 |
Sentence |
denotes |
Also TEM experiments of gold nanoparticles coated with the pyruvated trisaccharide could not make visible aggregation in the presence of Ca(2+)-ions. |
TextSentencer_T12 |
1984-2128 |
Sentence |
denotes |
It is suggested that the self-interaction between the sulfated disaccharide fragments is stronger than that between the pyruvated trisaccharide. |
T12 |
1984-2128 |
Sentence |
denotes |
It is suggested that the self-interaction between the sulfated disaccharide fragments is stronger than that between the pyruvated trisaccharide. |