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PubMed:18782853 JSONTXT 65 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-118 Sentence denotes Screening a limited structure-based library identifies UDP-GalNAc-specific mutants of alpha-1,3-galactosyltransferase.
T1 0-118 Sentence denotes Screening a limited structure-based library identifies UDP-GalNAc-specific mutants of alpha-1,3-galactosyltransferase.
T1 0-118 Sentence denotes Screening a limited structure-based library identifies UDP-GalNAc-specific mutants of alpha-1,3-galactosyltransferase.
TextSentencer_T2 119-234 Sentence denotes Complex glycans have important roles in biological recognition processes and considerable pharmaceutical potential.
T2 119-234 Sentence denotes Complex glycans have important roles in biological recognition processes and considerable pharmaceutical potential.
T2 119-234 Sentence denotes Complex glycans have important roles in biological recognition processes and considerable pharmaceutical potential.
TextSentencer_T3 235-361 Sentence denotes The synthesis of novel glycans can be facilitated by engineering glycosyltransferases to modify their substrate specificities.
T3 235-361 Sentence denotes The synthesis of novel glycans can be facilitated by engineering glycosyltransferases to modify their substrate specificities.
T3 235-361 Sentence denotes The synthesis of novel glycans can be facilitated by engineering glycosyltransferases to modify their substrate specificities.
TextSentencer_T4 362-575 Sentence denotes The choice of sites to modify requires the knowledge of the structures of enzyme-substrate complexes while the complexity of protein structures necessitates the exploration of a large array of multisite mutations.
T4 362-575 Sentence denotes The choice of sites to modify requires the knowledge of the structures of enzyme-substrate complexes while the complexity of protein structures necessitates the exploration of a large array of multisite mutations.
T4 362-575 Sentence denotes The choice of sites to modify requires the knowledge of the structures of enzyme-substrate complexes while the complexity of protein structures necessitates the exploration of a large array of multisite mutations.
TextSentencer_T5 576-773 Sentence denotes The retaining glycosyltransferase, alpha-1,3-galactosyltransferase (alpha3GT), which catalyzes the synthesis of the alpha-Gal epitope, has strict specificity for UDP-galactose as a donor substrate.
T5 576-773 Sentence denotes The retaining glycosyltransferase, alpha-1,3-galactosyltransferase (alpha3GT), which catalyzes the synthesis of the alpha-Gal epitope, has strict specificity for UDP-galactose as a donor substrate.
T5 576-773 Sentence denotes The retaining glycosyltransferase, alpha-1,3-galactosyltransferase (alpha3GT), which catalyzes the synthesis of the alpha-Gal epitope, has strict specificity for UDP-galactose as a donor substrate.
TextSentencer_T6 774-996 Sentence denotes Based on the structure of a complex of UDP-galactose with alpha3GT, the specificity for the galactose moiety can be partly attributed to residues that interact with the galactose 2-OH group, particularly His280 and Ala282.
T6 774-996 Sentence denotes Based on the structure of a complex of UDP-galactose with alpha3GT, the specificity for the galactose moiety can be partly attributed to residues that interact with the galactose 2-OH group, particularly His280 and Ala282.
T6 774-996 Sentence denotes Based on the structure of a complex of UDP-galactose with alpha3GT, the specificity for the galactose moiety can be partly attributed to residues that interact with the galactose 2-OH group, particularly His280 and Ala282.
TextSentencer_T7 997-1249 Sentence denotes With the goal of engineering a variant of bovine alpha3GT with GalNAc transferase activity, we constructed a limited library of 456 alpha3GT mutants containing 19 alternative amino acids at position 280, two each at 281 and 282 and six at position 283.
T7 997-1249 Sentence denotes With the goal of engineering a variant of bovine alpha3GT with GalNAc transferase activity, we constructed a limited library of 456 alpha3GT mutants containing 19 alternative amino acids at position 280, two each at 281 and 282 and six at position 283.
T7 997-1249 Sentence denotes With the goal of engineering a variant of bovine alpha3GT with GalNAc transferase activity, we constructed a limited library of 456 alpha3GT mutants containing 19 alternative amino acids at position 280, two each at 281 and 282 and six at position 283.
TextSentencer_T8 1250-1372 Sentence denotes Clones (1500) were screened by assaying partially purified bacterially expressed variants for GalNAc transferase activity.
T8 1250-1372 Sentence denotes Clones (1500) were screened by assaying partially purified bacterially expressed variants for GalNAc transferase activity.
T8 1250-1372 Sentence denotes Clones (1500) were screened by assaying partially purified bacterially expressed variants for GalNAc transferase activity.
TextSentencer_T9 1373-1487 Sentence denotes Mutants with the highest levels of GalNAc transferase activity, AGGL or GGGL, had substitutions at all four sites.
T9 1373-1487 Sentence denotes Mutants with the highest levels of GalNAc transferase activity, AGGL or GGGL, had substitutions at all four sites.
T9 1373-1487 Sentence denotes Mutants with the highest levels of GalNAc transferase activity, AGGL or GGGL, had substitutions at all four sites.
TextSentencer_T10 1488-1629 Sentence denotes The AGGL mutant had slightly superior GalNAc transferase activity amounting to about 3% of the activity of the wild-type enzyme with UDP-Gal.
T10 1488-1629 Sentence denotes The AGGL mutant had slightly superior GalNAc transferase activity amounting to about 3% of the activity of the wild-type enzyme with UDP-Gal.
T10 1488-1830 Sentence denotes The AGGL mutant had slightly superior GalNAc transferase activity amounting to about 3% of the activity of the wild-type enzyme with UDP-Gal. This mutant had a low activity with UDP-Gal; its crystallographic structure suggests that the smaller side chains at residues 280-282 form a pocket to accommodate the larger acetamido group of GalNAc.
TextSentencer_T11 1630-1830 Sentence denotes This mutant had a low activity with UDP-Gal; its crystallographic structure suggests that the smaller side chains at residues 280-282 form a pocket to accommodate the larger acetamido group of GalNAc.
T11 1630-1830 Sentence denotes This mutant had a low activity with UDP-Gal; its crystallographic structure suggests that the smaller side chains at residues 280-282 form a pocket to accommodate the larger acetamido group of GalNAc.
TextSentencer_T12 1831-1913 Sentence denotes Mutational studies indicate that Leu283 is important for stability in this mutant.
T11 1831-1913 Sentence denotes Mutational studies indicate that Leu283 is important for stability in this mutant.
T12 1831-1913 Sentence denotes Mutational studies indicate that Leu283 is important for stability in this mutant.