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PubMed:18285341 JSONTXT 38 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-115 Sentence denotes Distinct effects of N-acetylgalactosamine-4-sulfatase and galactose-6-sulfatase expression on chondroitin sulfates.
T2 116-262 Sentence denotes The sulfatase enzymes, N-acetylgalactosamine-4-sulfatase (arylsulfatase B (ASB)) and galactose-6-sulfatase (GALNS) hydrolyze sulfate groups of CS.
T3 263-339 Sentence denotes Deficiencies of ASB and GALNS are associated with the mucopolysaccharidoses.
T4 340-618 Sentence denotes To determine if expression of ASB and GALNS impacts on glycosaminoglycans (GAGs) and proteoglycans beyond their association with the mucopolysaccharidoses, we modified the expression of ASB and GALNS by overexpression and by silencing with small interference RNA in MCF-7 cells.
T5 619-831 Sentence denotes Content of total sulfated GAG (sGAG), chondroitin 4-sulfate (C4S), and total chondroitin sulfates (CSs) was measured following immunoprecipitation with C4S and CS antibodies and treatment with chondroitinase ABC.
T6 832-917 Sentence denotes Following silencing of ASB or GALNS, total sGAG, C4S, and CS increased significantly.
T7 918-1007 Sentence denotes Following overexpression of ASB or GALNS, total sGAG, C4S, and CS declined significantly.
T8 1008-1211 Sentence denotes Measurements following chondroitinase ABC treatment of the cell lysates demonstrated no change in the content of the other sGAG, including heparin, heparan sulfate, dermatan sulfate, and keratan sulfate.
T9 1212-1320 Sentence denotes Following overexpression of ASB and immunoprecipitation with C4S antibody, virtually no sGAG was detectable.
T10 1321-1635 Sentence denotes Total sGAG content increased to 23.39 (+/-1.06) microg/mg of protein from baseline of 12.47 (+/-0.68) microg/mg of protein following ASB silencing. mRNA expression of core proteins of the CS-containing proteoglycans, syndecan-1 and decorin, was significantly up-regulated following overexpression of ASB and GALNS.
T11 1636-1770 Sentence denotes Soluble syndecan-1 protein increased following increases in ASB and GALNS and reduced following silencing, inversely to changes in CS.
T12 1771-1901 Sentence denotes These findings demonstrate that modification of expression of the lysosomal sulfatases ASB and GALNS regulates the content of CSs.