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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-112 Sentence denotes Purification and characterization of avian beta 1,4 galactosyltransferase: comparison with the mammalian enzyme.
T1 0-112 Sentence denotes Purification and characterization of avian beta 1,4 galactosyltransferase: comparison with the mammalian enzyme.
T1 0-112 Sentence denotes Purification and characterization of avian beta 1,4 galactosyltransferase: comparison with the mammalian enzyme.
TextSentencer_T2 113-345 Sentence denotes Avian beta 1,4 galactosyltransferase (GalTase) was purified from chicken serum, partially characterized and compared to mammalian GalTase using antibody cross-reactivity, Northern blot hybridization and amino acid sequence analysis.
T2 113-345 Sentence denotes Avian beta 1,4 galactosyltransferase (GalTase) was purified from chicken serum, partially characterized and compared to mammalian GalTase using antibody cross-reactivity, Northern blot hybridization and amino acid sequence analysis.
T2 113-345 Sentence denotes Avian beta 1,4 galactosyltransferase (GalTase) was purified from chicken serum, partially characterized and compared to mammalian GalTase using antibody cross-reactivity, Northern blot hybridization and amino acid sequence analysis.
TextSentencer_T3 346-591 Sentence denotes The enzyme was purified to apparent homogeneity by alpha-lactalbumin(LA)-agarose affinity chromatography followed by preparative SDS-polyacrylamide gel electrophoresis, and identified as two proteins of apparent molecular masses of 39 and 46 kD.
T3 346-591 Sentence denotes The enzyme was purified to apparent homogeneity by alpha-lactalbumin(LA)-agarose affinity chromatography followed by preparative SDS-polyacrylamide gel electrophoresis, and identified as two proteins of apparent molecular masses of 39 and 46 kD.
T3 346-591 Sentence denotes The enzyme was purified to apparent homogeneity by alpha-lactalbumin(LA)-agarose affinity chromatography followed by preparative SDS-polyacrylamide gel electrophoresis, and identified as two proteins of apparent molecular masses of 39 and 46 kD.
TextSentencer_T4 592-727 Sentence denotes Chicken serum GalTase had a Km for UDPGal of 42 microM, for GlcNAc of 10 mM and had optimal activity in the presence of 10-20 mM MnCl2.
T4 592-727 Sentence denotes Chicken serum GalTase had a Km for UDPGal of 42 microM, for GlcNAc of 10 mM and had optimal activity in the presence of 10-20 mM MnCl2.
T4 592-727 Sentence denotes Chicken serum GalTase had a Km for UDPGal of 42 microM, for GlcNAc of 10 mM and had optimal activity in the presence of 10-20 mM MnCl2.
TextSentencer_T5 728-1150 Sentence denotes Substrate and linkage specificity analyses indicated that the purified enzyme behaves as a traditional Gal beta 1,4 GlcNAc:GalTase, since: (i) the avian beta 1,4 GalTase bound to alpha-LA; (ii) terminal GlcNAc residues served as good acceptors for chicken serum GalTase; (iii) the enzyme was inhibited by high concentrations of GlcNAc; (iv) the galactosylated product was sensitive to beta 1,4-specific beta-galactosidase.
T5 728-1150 Sentence denotes Substrate and linkage specificity analyses indicated that the purified enzyme behaves as a traditional Gal beta 1,4 GlcNAc:GalTase, since: (i) the avian beta 1,4 GalTase bound to alpha-LA; (ii) terminal GlcNAc residues served as good acceptors for chicken serum GalTase; (iii) the enzyme was inhibited by high concentrations of GlcNAc; (iv) the galactosylated product was sensitive to beta 1,4-specific beta-galactosidase.
T5 728-1150 Sentence denotes Substrate and linkage specificity analyses indicated that the purified enzyme behaves as a traditional Gal beta 1,4 GlcNAc:GalTase, since: (i) the avian beta 1,4 GalTase bound to alpha-LA; (ii) terminal GlcNAc residues served as good acceptors for chicken serum GalTase; (iii) the enzyme was inhibited by high concentrations of GlcNAc; (iv) the galactosylated product was sensitive to beta 1,4-specific beta-galactosidase.
TextSentencer_T6 1151-1258 Sentence denotes Finally, the disaccharide reaction product comigrated with authentic beta 1,4 N-acetyllactosamine standard.
T6 1151-1258 Sentence denotes Finally, the disaccharide reaction product comigrated with authentic beta 1,4 N-acetyllactosamine standard.
T6 1151-1258 Sentence denotes Finally, the disaccharide reaction product comigrated with authentic beta 1,4 N-acetyllactosamine standard.
TextSentencer_T7 1259-1351 Sentence denotes No other GalTase activities were detectable using a battery of defined glycoside substrates.
T7 1259-1351 Sentence denotes No other GalTase activities were detectable using a battery of defined glycoside substrates.
T7 1259-1351 Sentence denotes No other GalTase activities were detectable using a battery of defined glycoside substrates.
TextSentencer_T8 1352-1504 Sentence denotes Polyclonal antibodies raised against the two gel-purified GalTase proteins showed reactivity with avian GalTase by ELISA and immunoprecipitation assays.
T8 1352-1504 Sentence denotes Polyclonal antibodies raised against the two gel-purified GalTase proteins showed reactivity with avian GalTase by ELISA and immunoprecipitation assays.
T8 1352-1504 Sentence denotes Polyclonal antibodies raised against the two gel-purified GalTase proteins showed reactivity with avian GalTase by ELISA and immunoprecipitation assays.
TextSentencer_T9 1505-1616 Sentence denotes The antibodies also inhibited GalTase activity toward both high mol. wt and monosaccharide acceptor substrates.
T9 1505-1616 Sentence denotes The antibodies also inhibited GalTase activity toward both high mol. wt and monosaccharide acceptor substrates.
T9 1505-1616 Sentence denotes The antibodies also inhibited GalTase activity toward both high mol. wt and monosaccharide acceptor substrates.
TextSentencer_T10 1617-1933 Sentence denotes Despite similar kinetics and substrate specificity, the avian and mammalian GalTases showed little overall structural similarity, since polyclonal anti-avian GalTase IgG failed to react with mammalian GalTase purified from bovine milk, and conversely anti-bovine milk GalTase IgG did not react with the avian enzyme.
T10 1617-1933 Sentence denotes Despite similar kinetics and substrate specificity, the avian and mammalian GalTases showed little overall structural similarity, since polyclonal anti-avian GalTase IgG failed to react with mammalian GalTase purified from bovine milk, and conversely anti-bovine milk GalTase IgG did not react with the avian enzyme.
T10 1617-1933 Sentence denotes Despite similar kinetics and substrate specificity, the avian and mammalian GalTases showed little overall structural similarity, since polyclonal anti-avian GalTase IgG failed to react with mammalian GalTase purified from bovine milk, and conversely anti-bovine milk GalTase IgG did not react with the avian enzyme.
TextSentencer_T11 1934-2125 Sentence denotes Furthermore, in Northern blot analysis, no hybridization was detected when chicken embryo liver poly(A)+ RNA was probed with a mouse GalTase cDNA, even under conditions of reduced stringency.
T11 1934-2125 Sentence denotes Furthermore, in Northern blot analysis, no hybridization was detected when chicken embryo liver poly(A)+ RNA was probed with a mouse GalTase cDNA, even under conditions of reduced stringency.
T11 1934-2125 Sentence denotes Furthermore, in Northern blot analysis, no hybridization was detected when chicken embryo liver poly(A)+ RNA was probed with a mouse GalTase cDNA, even under conditions of reduced stringency.
TextSentencer_T12 2126-2332 Sentence denotes Amino acid sequence analysis identified three of five tryptic peptides that are homologous to the mammalian sequence within a putative substrate binding domain and the carboxy terminal domain of the enzyme.
T12 2126-2332 Sentence denotes Amino acid sequence analysis identified three of five tryptic peptides that are homologous to the mammalian sequence within a putative substrate binding domain and the carboxy terminal domain of the enzyme.
T12 2126-2332 Sentence denotes Amino acid sequence analysis identified three of five tryptic peptides that are homologous to the mammalian sequence within a putative substrate binding domain and the carboxy terminal domain of the enzyme.
TextSentencer_T13 2333-2495 Sentence denotes Their overall structural disparity leads us to believe that regions of homology between the avian and mammalian GalTases may represent active sites of the enzyme.
T13 2333-2495 Sentence denotes Their overall structural disparity leads us to believe that regions of homology between the avian and mammalian GalTases may represent active sites of the enzyme.
T13 2333-2495 Sentence denotes Their overall structural disparity leads us to believe that regions of homology between the avian and mammalian GalTases may represent active sites of the enzyme.