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Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-133 Sentence denotes Glycoprotein biosynthesis in yeast: purification and characterization of the endoplasmic reticulum Man9 processing alpha-mannosidase.
T1 0-133 Sentence denotes Glycoprotein biosynthesis in yeast: purification and characterization of the endoplasmic reticulum Man9 processing alpha-mannosidase.
T1 0-133 Sentence denotes Glycoprotein biosynthesis in yeast: purification and characterization of the endoplasmic reticulum Man9 processing alpha-mannosidase.
TextSentencer_T2 134-419 Sentence denotes Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion.
T2 134-419 Sentence denotes Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion.
T2 134-419 Sentence denotes Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion.
TextSentencer_T3 420-599 Sentence denotes The activity was solubilized by the zwitterionic detergent, 3-[(3-cholamidopropyl)dimethyl ammonio]-1-propanesulphonate (CHAPS), whose presence was necessary for maximal activity.
T3 420-599 Sentence denotes The activity was solubilized by the zwitterionic detergent, 3-[(3-cholamidopropyl)dimethyl ammonio]-1-propanesulphonate (CHAPS), whose presence was necessary for maximal activity.
T3 420-599 Sentence denotes The activity was solubilized by the zwitterionic detergent, 3-[(3-cholamidopropyl)dimethyl ammonio]-1-propanesulphonate (CHAPS), whose presence was necessary for maximal activity.
TextSentencer_T4 600-739 Sentence denotes Purification included Q-Sepharose ion-exchange chromatography, preparative isoelectric focusing and HPLC gel filtration on TSK 3000 matrix.
T4 600-739 Sentence denotes Purification included Q-Sepharose ion-exchange chromatography, preparative isoelectric focusing and HPLC gel filtration on TSK 3000 matrix.
T4 600-739 Sentence denotes Purification included Q-Sepharose ion-exchange chromatography, preparative isoelectric focusing and HPLC gel filtration on TSK 3000 matrix.
TextSentencer_T5 740-857 Sentence denotes Overall purification from post-nuclear supernatants was estimated to be 110,000-fold with a 50% recovery of activity.
T5 740-857 Sentence denotes Overall purification from post-nuclear supernatants was estimated to be 110,000-fold with a 50% recovery of activity.
T5 740-857 Sentence denotes Overall purification from post-nuclear supernatants was estimated to be 110,000-fold with a 50% recovery of activity.
TextSentencer_T6 858-1047 Sentence denotes The purified enzyme hydrolysed Man9GlcNAc1,2 from thyroglobulin or oligosaccharide-lipid, but not invertase Man9GlcNAc, Man1 alpha 2Man1 alpha OCH3 or p-nitrophenyl-alpha-D-mannopyranoside.
T6 858-1047 Sentence denotes The purified enzyme hydrolysed Man9GlcNAc1,2 from thyroglobulin or oligosaccharide-lipid, but not invertase Man9GlcNAc, Man1 alpha 2Man1 alpha OCH3 or p-nitrophenyl-alpha-D-mannopyranoside.
T6 858-1047 Sentence denotes The purified enzyme hydrolysed Man9GlcNAc1,2 from thyroglobulin or oligosaccharide-lipid, but not invertase Man9GlcNAc, Man1 alpha 2Man1 alpha OCH3 or p-nitrophenyl-alpha-D-mannopyranoside.
TextSentencer_T7 1048-1215 Sentence denotes Conversion of thyroglobulin Man9GlcNAc to Man8GlcNAc was linear with time and enzyme concentration, with an apparent Km of 0.2 mM and a specific activity of 220 IU/mg.
T7 1048-1215 Sentence denotes Conversion of thyroglobulin Man9GlcNAc to Man8GlcNAc was linear with time and enzyme concentration, with an apparent Km of 0.2 mM and a specific activity of 220 IU/mg.
T7 1048-1215 Sentence denotes Conversion of thyroglobulin Man9GlcNAc to Man8GlcNAc was linear with time and enzyme concentration, with an apparent Km of 0.2 mM and a specific activity of 220 IU/mg.
TextSentencer_T8 1216-1378 Sentence denotes Glc3Man9GlcNAc2 from oligosaccharide-lipid was as good a substrate as Man9GlcNAc, but the lipid-linked Man7GlcNAc2 isomer was hydrolysed at only 10% of this rate.
T8 1216-1378 Sentence denotes Glc3Man9GlcNAc2 from oligosaccharide-lipid was as good a substrate as Man9GlcNAc, but the lipid-linked Man7GlcNAc2 isomer was hydrolysed at only 10% of this rate.
T8 1216-1378 Sentence denotes Glc3Man9GlcNAc2 from oligosaccharide-lipid was as good a substrate as Man9GlcNAc, but the lipid-linked Man7GlcNAc2 isomer was hydrolysed at only 10% of this rate.
TextSentencer_T9 1379-1627 Sentence denotes Hydrolysis of defined isomers of IgM and bovine thyroglobulin Man6,7,8GlcNAc indicated that, for maximal alpha 1,2-mannosidase activity, only the alpha 1,2-linked terminal mannoses on the alpha 3 branch of the Man9GlcNAc precursor were dispensable.
T9 1379-1627 Sentence denotes Hydrolysis of defined isomers of IgM and bovine thyroglobulin Man6,7,8GlcNAc indicated that, for maximal alpha 1,2-mannosidase activity, only the alpha 1,2-linked terminal mannoses on the alpha 3 branch of the Man9GlcNAc precursor were dispensable.
T9 1379-1627 Sentence denotes Hydrolysis of defined isomers of IgM and bovine thyroglobulin Man6,7,8GlcNAc indicated that, for maximal alpha 1,2-mannosidase activity, only the alpha 1,2-linked terminal mannoses on the alpha 3 branch of the Man9GlcNAc precursor were dispensable.
TextSentencer_T10 1628-1766 Sentence denotes Isomers lacking the terminal alpha 1,2-linked mannose on the alpha 6 branch were hydrolysed at only approximately 10% of the maximal rate.
T10 1628-1766 Sentence denotes Isomers lacking the terminal alpha 1,2-linked mannose on the alpha 6 branch were hydrolysed at only approximately 10% of the maximal rate.
T10 1628-1766 Sentence denotes Isomers lacking the terminal alpha 1,2-linked mannose on the alpha 6 branch were hydrolysed at only approximately 10% of the maximal rate.
TextSentencer_T11 1767-1824 Sentence denotes The enzyme exhibited a pI of 5.3 and a pH optimum at 6.5.
T11 1767-1824 Sentence denotes The enzyme exhibited a pI of 5.3 and a pH optimum at 6.5.
T11 1767-1824 Sentence denotes The enzyme exhibited a pI of 5.3 and a pH optimum at 6.5.
TextSentencer_T12 1825-2082 Sentence denotes Sodium dodecyl sulphate-polyacrylamide gel electrophoresis in the absence of reducing agents gave a single sharp band at 66 kDa, while in the presence of beta-mercaptoethanol equimolar amounts of two peptides, one of 44 kDa and one of 23 kDa, were obtained.
T12 1825-2082 Sentence denotes Sodium dodecyl sulphate-polyacrylamide gel electrophoresis in the absence of reducing agents gave a single sharp band at 66 kDa, while in the presence of beta-mercaptoethanol equimolar amounts of two peptides, one of 44 kDa and one of 23 kDa, were obtained.
T12 1825-2082 Sentence denotes Sodium dodecyl sulphate-polyacrylamide gel electrophoresis in the absence of reducing agents gave a single sharp band at 66 kDa, while in the presence of beta-mercaptoethanol equimolar amounts of two peptides, one of 44 kDa and one of 23 kDa, were obtained.
TextSentencer_T13 2083-2320 Sentence denotes Sizing on Sephacryl SF300, Superose 12 and TSK 3000 provided a holoenzyme mol. wt of 60-68 kDa, indicating that the isolated active form of the Man9-alpha-mannosidase was composed of one each of the sulphydryl-bonded dissimilar peptides.
T13 2083-2320 Sentence denotes Sizing on Sephacryl SF300, Superose 12 and TSK 3000 provided a holoenzyme mol. wt of 60-68 kDa, indicating that the isolated active form of the Man9-alpha-mannosidase was composed of one each of the sulphydryl-bonded dissimilar peptides.
T13 2083-2320 Sentence denotes Sizing on Sephacryl SF300, Superose 12 and TSK 3000 provided a holoenzyme mol. wt of 60-68 kDa, indicating that the isolated active form of the Man9-alpha-mannosidase was composed of one each of the sulphydryl-bonded dissimilar peptides.
TextSentencer_T14 2321-2473 Sentence denotes The enzyme bound to concanavalin A (ConA)-Sepharose and was eluted with alpha-methylmannoside, indicating the presence of high-mannose oligosaccharides.
T14 2321-2473 Sentence denotes The enzyme bound to concanavalin A (ConA)-Sepharose and was eluted with alpha-methylmannoside, indicating the presence of high-mannose oligosaccharides.
T14 2321-2473 Sentence denotes The enzyme bound to concanavalin A (ConA)-Sepharose and was eluted with alpha-methylmannoside, indicating the presence of high-mannose oligosaccharides.
TextSentencer_T15 2474-2561 Sentence denotes The Man9-alpha-mannosidase required low levels of Ca2+, which could be removed by EGTA.
T15 2474-2561 Sentence denotes The Man9-alpha-mannosidase required low levels of Ca2+, which could be removed by EGTA.
T15 2474-2561 Sentence denotes The Man9-alpha-mannosidase required low levels of Ca2+, which could be removed by EGTA.
TextSentencer_T16 2562-2625 Sentence denotes Activity was restored by Ca2+ or Zn2+, but not by Mg2+ or Mn2+.
T16 2562-2625 Sentence denotes Activity was restored by Ca2+ or Zn2+, but not by Mg2+ or Mn2+.
T16 2562-2625 Sentence denotes Activity was restored by Ca2+ or Zn2+, but not by Mg2+ or Mn2+.