| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-128 |
Sentence |
denotes |
Characterization of O-linked oligosaccharide biosynthesis in cultured cells using paranitrophenyl alpha-D-GalNAc as an acceptor. |
| T1 |
0-128 |
Sentence |
denotes |
Characterization of O-linked oligosaccharide biosynthesis in cultured cells using paranitrophenyl alpha-D-GalNAc as an acceptor. |
| T1 |
0-128 |
Sentence |
denotes |
Characterization of O-linked oligosaccharide biosynthesis in cultured cells using paranitrophenyl alpha-D-GalNAc as an acceptor. |
| TextSentencer_T2 |
129-367 |
Sentence |
denotes |
Aryl-N-acetyl-alpha-galactosaminides (aryl-GalNAc) are acceptor substrates for UDP-Gal:alpha-GalNAc beta 1-3 galactosyltransferase and, in vivo, aryl-GalNAc have been shown to inhibit O-linked oligosaccharide biosynthesis (Kuan et al., J. |
| T2 |
129-367 |
Sentence |
denotes |
Aryl-N-acetyl-alpha-galactosaminides (aryl-GalNAc) are acceptor substrates for UDP-Gal:alpha-GalNAc beta 1-3 galactosyltransferase and, in vivo, aryl-GalNAc have been shown to inhibit O-linked oligosaccharide biosynthesis (Kuan et al., J. |
| T2 |
129-398 |
Sentence |
denotes |
Aryl-N-acetyl-alpha-galactosaminides (aryl-GalNAc) are acceptor substrates for UDP-Gal:alpha-GalNAc beta 1-3 galactosyltransferase and, in vivo, aryl-GalNAc have been shown to inhibit O-linked oligosaccharide biosynthesis (Kuan et al., J. Biol. Chem. 264, 19271, 1989). |
| TextSentencer_T3 |
368-373 |
Sentence |
denotes |
Biol. |
| T3 |
368-373 |
Sentence |
denotes |
Biol. |
| TextSentencer_T4 |
374-379 |
Sentence |
denotes |
Chem. |
| T4 |
374-379 |
Sentence |
denotes |
Chem. |
| TextSentencer_T5 |
380-398 |
Sentence |
denotes |
264, 19271, 1989). |
| T5 |
380-398 |
Sentence |
denotes |
264, 19271, 1989). |
| TextSentencer_T6 |
399-638 |
Sentence |
denotes |
Since aryl-GalNAc, appears to enter viable cells and serve as an acceptor for O-glycosylation enzymes, the recovery and characterization of the aryl-oligosaccharides from cell culture medium may reflect cellular pattems of O-glycosylation. |
| T3 |
399-638 |
Sentence |
denotes |
Since aryl-GalNAc, appears to enter viable cells and serve as an acceptor for O-glycosylation enzymes, the recovery and characterization of the aryl-oligosaccharides from cell culture medium may reflect cellular pattems of O-glycosylation. |
| T6 |
399-638 |
Sentence |
denotes |
Since aryl-GalNAc, appears to enter viable cells and serve as an acceptor for O-glycosylation enzymes, the recovery and characterization of the aryl-oligosaccharides from cell culture medium may reflect cellular pattems of O-glycosylation. |
| TextSentencer_T7 |
639-789 |
Sentence |
denotes |
To pursue this possibility, the following paranitrophenyl-linked oligosaccharide standards were enzymatically synthesized and characterized by 1H-NMR: |
| T4 |
639-789 |
Sentence |
denotes |
To pursue this possibility, the following paranitrophenyl-linked oligosaccharide standards were enzymatically synthesized and characterized by 1H-NMR: |
| T7 |
639-789 |
Sentence |
denotes |
To pursue this possibility, the following paranitrophenyl-linked oligosaccharide standards were enzymatically synthesized and characterized by 1H-NMR: |
| TextSentencer_T8 |
790-1021 |
Sentence |
denotes |
Gal beta 1-3(GlcNAc beta 1-6)Gal-NAc alpha-pNp; Gal beta 1-3(Gal beta 1-4GlcNAc beta 1-6)GalNAc alpha-pNp; SA alpha 2-3Gal beta 1-3(SA alpha 2-3Gal beta 1-4GlcNAc,beta 1-6)GalNAc alpha-pNp; SA alpha 2-3Gal beta 1-3GalNAc alpha-pNp. |
| T5 |
790-1021 |
Sentence |
denotes |
Gal beta 1-3(GlcNAc beta 1-6)Gal-NAc alpha-pNp; Gal beta 1-3(Gal beta 1-4GlcNAc beta 1-6)GalNAc alpha-pNp; SA alpha 2-3Gal beta 1-3(SA alpha 2-3Gal beta 1-4GlcNAc,beta 1-6)GalNAc alpha-pNp; SA alpha 2-3Gal beta 1-3GalNAc alpha-pNp. |
| T8 |
790-1021 |
Sentence |
denotes |
Gal beta 1-3(GlcNAc beta 1-6)Gal-NAc alpha-pNp; Gal beta 1-3(Gal beta 1-4GlcNAc beta 1-6)GalNAc alpha-pNp; SA alpha 2-3Gal beta 1-3(SA alpha 2-3Gal beta 1-4GlcNAc,beta 1-6)GalNAc alpha-pNp; SA alpha 2-3Gal beta 1-3GalNAc alpha-pNp. |
| TextSentencer_T9 |
1022-1148 |
Sentence |
denotes |
As a model system, MDAY-D2 lymphoid tumour cells were cultured for various periods in medium containing 2 mM GalNAc alpha-pNp. |
| T6 |
1022-1148 |
Sentence |
denotes |
As a model system, MDAY-D2 lymphoid tumour cells were cultured for various periods in medium containing 2 mM GalNAc alpha-pNp. |
| T9 |
1022-1148 |
Sentence |
denotes |
As a model system, MDAY-D2 lymphoid tumour cells were cultured for various periods in medium containing 2 mM GalNAc alpha-pNp. |
| TextSentencer_T10 |
1149-1341 |
Sentence |
denotes |
The secreted aryl-oligosaccharides were separated by Biogel P2 chromatography and DEAE HPLC, followed by further fractionation of the disialyl oligosaccharides on an Ultrahydrogel HPLC column. |
| T7 |
1149-1341 |
Sentence |
denotes |
The secreted aryl-oligosaccharides were separated by Biogel P2 chromatography and DEAE HPLC, followed by further fractionation of the disialyl oligosaccharides on an Ultrahydrogel HPLC column. |
| T10 |
1149-1341 |
Sentence |
denotes |
The secreted aryl-oligosaccharides were separated by Biogel P2 chromatography and DEAE HPLC, followed by further fractionation of the disialyl oligosaccharides on an Ultrahydrogel HPLC column. |
| TextSentencer_T11 |
1342-1517 |
Sentence |
denotes |
Absorbance of the paranitrophenyl aryl constituent at 303 nm allowed detection at the 10 pmol level and provided a relatively specific means of following the oligosaccharides. |
| T8 |
1342-1517 |
Sentence |
denotes |
Absorbance of the paranitrophenyl aryl constituent at 303 nm allowed detection at the 10 pmol level and provided a relatively specific means of following the oligosaccharides. |
| T11 |
1342-1517 |
Sentence |
denotes |
Absorbance of the paranitrophenyl aryl constituent at 303 nm allowed detection at the 10 pmol level and provided a relatively specific means of following the oligosaccharides. |
| TextSentencer_T12 |
1518-1791 |
Sentence |
denotes |
MDAY-D2 cells produced disialylated aryl-oligosaccharides at a rate of 20 pmol/h/10(6) cells with a half-time of transit to the cell surface of 13.6 min, a rate consistent with their movement from the Golgi to the cell surface by bulk flow.(ABSTRACT TRUNCATED AT 250 WORDS) |
| T9 |
1518-1791 |
Sentence |
denotes |
MDAY-D2 cells produced disialylated aryl-oligosaccharides at a rate of 20 pmol/h/10(6) cells with a half-time of transit to the cell surface of 13.6 min, a rate consistent with their movement from the Golgi to the cell surface by bulk flow.(ABSTRACT TRUNCATED AT 250 WORDS) |
| T12 |
1518-1791 |
Sentence |
denotes |
MDAY-D2 cells produced disialylated aryl-oligosaccharides at a rate of 20 pmol/h/10(6) cells with a half-time of transit to the cell surface of 13.6 min, a rate consistent with their movement from the Golgi to the cell surface by bulk flow.(ABSTRACT TRUNCATED AT 250 WORDS) |