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PubMed:18174169 JSONTXT 38 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-131 Sentence denotes Glucose deprivation stimulates O-GlcNAc modification of proteins through up-regulation of O-linked N-acetylglucosaminyltransferase.
T2 132-268 Sentence denotes O-Linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of proteins that functions as a nutrient sensing mechanism.
T3 269-362 Sentence denotes Here we report on regulation of O-GlcNAcylation over a broad range of glucose concentrations.
T4 363-503 Sentence denotes We have discovered a significant induction of O-GlcNAc modification of a limited number of proteins under conditions of glucose deprivation.
T5 504-726 Sentence denotes Beginning 12 h after treatment, glucose-deprived human hepatocellular carcinoma (HepG2) cells demonstrate a 7.8-fold increase in total O-GlcNAc modification compared with cells cultured in normal glucose (5 mm; p = 0.008).
T6 727-975 Sentence denotes Some of the targets of glucose deprivation-induced O-GlcNAcylation are distinct from those modified in response to high glucose (20 mm) or glucosamine (10 mm) treatment, suggesting differential targeting with glucose deprivation and glucose excess.
T7 976-1165 Sentence denotes O-GlcNAcylation of glycogen synthase is significantly increased with glucose deprivation, and this O-GlcNAc increase contributes to a 60% decrease (p = 0.004) in glycogen synthase activity.
T8 1166-1287 Sentence denotes Increased O-GlcNAc modification is not mediated by increased UDP-GlcNAc, the rate-limiting substrate for O-GlcNAcylation.
T9 1288-1439 Sentence denotes Rather, the mRNA for nucleocytoplasmic O-linked N-acetylglucosaminyltransferase (OGT) increases 3.4-fold within 6 h of glucose deprivation (p = 0.006).
T10 1440-1538 Sentence denotes Within 12 h, OGT protein increases 1.7-fold (p = 0.01) compared with normal glucose-treated cells.
T11 1539-1642 Sentence denotes In addition, 12-h glucose deprivation leads to a 49% decrease in O-GlcNAcase protein levels (p = 0.03).
T12 1643-1869 Sentence denotes We conclude that increased O-GlcNAc modification stimulated by glucose deprivation results from increased OGT and decreased O-GlcNAcase levels and that these changes affect cell metabolism, thus inactivating glycogen synthase.