| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-71 |
Sentence |
denotes |
Identification of a biosynthetic gene cluster in rice for momilactones. |
| T2 |
72-279 |
Sentence |
denotes |
Rice diterpenoid phytoalexins such as momilactones and phytocassanes are produced in suspension-cultured rice cells treated with a chitin oligosaccharide elicitor and in rice leaves irradiated with UV light. |
| T3 |
280-486 |
Sentence |
denotes |
The common substrate geranylgeranyl diphosphate is converted into diterpene hydrocarbon precursors via a two-step sequential cyclization and then into the bioactive phytoalexins via several oxidation steps. |
| T4 |
487-730 |
Sentence |
denotes |
It has been suggested that microsomal cytochrome P-450 monooxygenases (P-450s) are involved in the downstream oxidation of the diterpene hydrocarbons leading to the phytoalexins and that a dehydrogenase is involved in momilactone biosynthesis. |
| T5 |
731-848 |
Sentence |
denotes |
However, none of the enzymes involved in the downstream oxidation of the diterpene hydrocarbons have been identified. |
| T6 |
849-1150 |
Sentence |
denotes |
In this study, we found that a putative dehydrogenase gene (AK103462) and two functionally unknown P-450 genes (CYP99A2 and CYP99A3) form a chitin oligosaccharide elicitor- and UV-inducible gene cluster, together with OsKS4 and OsCyc1, the diterpene cyclase genes involved in momilactone biosynthesis. |
| T7 |
1151-1383 |
Sentence |
denotes |
Functional analysis by heterologous expression in Escherichia coli followed by enzyme assays demonstrated that the AK103462 protein catalyzes the conversion of 3beta-hydroxy-9betaH-pimara-7,15-dien-19,6beta-olide into momilactone A. |
| T8 |
1384-1595 |
Sentence |
denotes |
The double knockdown of CYP99A2 and CYP99A3 specifically suppressed the elicitor-inducible production of momilactones, strongly suggesting that CYP99A2, CYP99A3, or both are involved in momilactone biosynthesis. |
| T9 |
1596-1722 |
Sentence |
denotes |
These results provide strong evidence for the presence on chromosome 4 of a gene cluster involved in momilactone biosynthesis. |
