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PubMed:17848574 JSONTXT 25 Projects

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Id Subject Object Predicate Lexical cue
T1 0-41 Sentence denotes MDM2 chaperones the p53 tumor suppressor.
T2 42-183 Sentence denotes The murine double minute (mdm2) gene encodes an E3 ubiquitin ligase that plays a key role in the degradation of p53 tumor suppressor protein.
T3 184-259 Sentence denotes Nevertheless recent data highlight other p53-independent functions of MDM2.
T4 260-609 Sentence denotes Given that MDM2 protein binds ATP, can interact with the Hsp90 chaperone, plays a role in the modulation of transcription factors and protection and activation of DNA polymerases, and is involved in ribosome assembly and nascent p53 protein biosynthesis, we have evaluated and found MDM2 protein to possess an intrinsic molecular chaperone activity.
T5 610-729 Sentence denotes MDM2 can substitute for the Hsp90 molecular chaperone in promoting binding of p53 to the p21-derived promoter sequence.
T6 730-833 Sentence denotes This reaction is driven by recycling of MDM2 from the p53 complex, triggered by binding of ATP to MDM2.
T7 834-933 Sentence denotes The ATP binding mutant MDM2 protein (K454A) lacks the chaperone activity both in vivo and in vitro.
T8 934-1092 Sentence denotes Mdm2 cotransfected in the H1299 cell line with wild-type p53 stimulates efficient p53 folding in vivo but at the same time accelerates the degradation of p53.
T9 1093-1222 Sentence denotes MDM2 in which one of the Zn(2+) coordinating residues is mutated (C478S or C464A) blocks degradation but enhances folding of p53.
T10 1223-1434 Sentence denotes This is the first demonstration that MDM2 possesses an intrinsic molecular chaperone activity, indicating that the ATP binding function of MDM2 can mediate its chaperone function toward the p53 tumor suppressor.