| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-90 |
Sentence |
denotes |
The yeast Hex3.Slx8 heterodimer is a ubiquitin ligase stimulated by substrate sumoylation. |
| T2 |
91-227 |
Sentence |
denotes |
Hex3 and Slx8 are Saccharomyces cerevisiae proteins with important functions in DNA damage control and maintenance of genomic stability. |
| T3 |
228-279 |
Sentence |
denotes |
Both proteins have RING domains at their C termini. |
| T4 |
280-428 |
Sentence |
denotes |
Such domains are common in ubiquitin and ubiquitin-like protein ligases (E3s), but little was known about the molecular functions of either protein. |
| T5 |
429-633 |
Sentence |
denotes |
In this study we identified HEX3 as a high-copy suppressor of a temperature-sensitive small ubiquitin-related modifier (SUMO) protease mutant, ulp1ts, suggesting that it may affect cellular SUMO dynamics. |
| T6 |
634-702 |
Sentence |
denotes |
Remarkably, even a complete deletion of ULP1 is strongly suppressed. |
| T7 |
703-738 |
Sentence |
denotes |
Hex3 forms a heterodimer with Slx8. |
| T8 |
739-836 |
Sentence |
denotes |
We found that the Hex3.Slx8 complex has a robust substrate-specific E3 ubiquitin ligase activity. |
| T9 |
837-946 |
Sentence |
denotes |
In this E3 complex, Slx8 appears to bear the core ligase function, with Hex3 strongly enhancing its activity. |
| T10 |
947-1110 |
Sentence |
denotes |
Notably, SUMO attachment to a substrate stimulates its Hex3.Slx8-dependent ubiquitination, primarily through direct noncovalent interactions between SUMO and Hex3. |
| T11 |
1111-1295 |
Sentence |
denotes |
Our data reveal a novel mechanism of substrate targeting in which sumoylation of a protein can help trigger its subsequent ubiquitination by recruiting a SUMO-binding ubiquitin ligase. |
