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PubMed:1718974 JSONTXT 28 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-91 Sentence denotes Functional insertion of the SV40 large T oncogene in cystic fibrosis intestinal epithelium.
T1 0-91 Sentence denotes Functional insertion of the SV40 large T oncogene in cystic fibrosis intestinal epithelium.
T2 92-124 Sentence denotes Characterization of CFI-3 cells.
T2 92-124 Sentence denotes Characterization of CFI-3 cells.
T3 125-284 Sentence denotes Intestinal epithelial cells were isolated from a fetus with cystic fibrosis (CF) and transfected with a plasmid vector recombined with the ori- mutant of SV40.
T3 125-284 Sentence denotes Intestinal epithelial cells were isolated from a fetus with cystic fibrosis (CF) and transfected with a plasmid vector recombined with the ori- mutant of SV40.
T4 285-364 Sentence denotes A population of proliferative cells was then subcloned and designated as CFI-3.
T4 285-364 Sentence denotes A population of proliferative cells was then subcloned and designated as CFI-3.
T5 365-458 Sentence denotes These cells had a doubling time of 24 h and were maintained in culture for up to 25 passages.
T5 365-458 Sentence denotes These cells had a doubling time of 24 h and were maintained in culture for up to 25 passages.
T6 459-525 Sentence denotes At passage 8, CFI-3 cells did not produce any tumors in nude mice.
T6 459-525 Sentence denotes At passage 8, CFI-3 cells did not produce any tumors in nude mice.
T7 526-663 Sentence denotes Northern blot and immunofluorescence studies indicated that the extended lifespan of CFI-3 cells results in genomic insertion of SV40 LT.
T7 526-663 Sentence denotes Northern blot and immunofluorescence studies indicated that the extended lifespan of CFI-3 cells results in genomic insertion of SV40 LT.
T8 664-832 Sentence denotes Intestinal CFI-3 cells are epithelial, according to the expression of the human cytokeratin 18 gene and poorly differentiated by phase-contrast and electron microscopy.
T8 664-832 Sentence denotes Intestinal CFI-3 cells are epithelial, according to the expression of the human cytokeratin 18 gene and poorly differentiated by phase-contrast and electron microscopy.
T9 833-1040 Sentence denotes Functional membrane receptors activated by vasoactive intestinal peptide (VIP), its natural analogue pituitary adenylate cyclase activating peptide (PACAP-38), and isoproterenol were observed in CFI-3 cells.
T9 833-1040 Sentence denotes Functional membrane receptors activated by vasoactive intestinal peptide (VIP), its natural analogue pituitary adenylate cyclase activating peptide (PACAP-38), and isoproterenol were observed in CFI-3 cells.
T10 1041-1198 Sentence denotes Restriction fragment length polymorphism analysis of the PstI KM19 site revealed that the cftr locus was identical in the chorionic villi and in CFI-3 cells.
T10 1041-1198 Sentence denotes Restriction fragment length polymorphism analysis of the PstI KM19 site revealed that the cftr locus was identical in the chorionic villi and in CFI-3 cells.
T11 1199-1362 Sentence denotes The manifestation of CF in this family was not related to the common mutation delta F508, since this fetus was heterozygous for the substitutions S549N and N1303K.
T11 1199-1362 Sentence denotes The manifestation of CF in this family was not related to the common mutation delta F508, since this fetus was heterozygous for the substitutions S549N and N1303K.
T12 1363-1622 Sentence denotes Chloride transport, assessed by the 125I efflux, was induced in CFI-3 cells by the calcium inophore ionomycin, but not by the adenylate cyclase activator forskolin, and was inhibited by the chloride channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid.
T12 1363-1622 Sentence denotes Chloride transport, assessed by the 125I efflux, was induced in CFI-3 cells by the calcium inophore ionomycin, but not by the adenylate cyclase activator forskolin, and was inhibited by the chloride channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid.
T13 1623-1810 Sentence denotes These results were confirmed in patch clamp studies in which the cpt cAMP analogue failed to stimulate membrane currents, while the calcium ionophore ionomycin stimulated inward currents.
T13 1623-1810 Sentence denotes These results were confirmed in patch clamp studies in which the cpt cAMP analogue failed to stimulate membrane currents, while the calcium ionophore ionomycin stimulated inward currents.
T14 1811-2124 Sentence denotes We conclude that intestinal CFI-3 cells retain the CF phenotype relating to defective regulation of Cl- channels, and therefore constitute a suitable model, 1) for elucidating the function of CFTR protein, 2) developing new therapeutic agents, and 3) correcting the CF defect by gene replacement therapy in vitro.
T14 1811-2124 Sentence denotes We conclude that intestinal CFI-3 cells retain the CF phenotype relating to defective regulation of Cl- channels, and therefore constitute a suitable model, 1) for elucidating the function of CFTR protein, 2) developing new therapeutic agents, and 3) correcting the CF defect by gene replacement therapy in vitro.