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PubMed:17137335 JSONTXT 42 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-176 Sentence denotes VDAC1, having a shorter N-terminus than VDAC2 but showing the same migration in an SDS-polyacrylamide gel, is the predominant form expressed in mitochondria of various tissues.
T1 0-176 Sentence denotes VDAC1, having a shorter N-terminus than VDAC2 but showing the same migration in an SDS-polyacrylamide gel, is the predominant form expressed in mitochondria of various tissues.
TextSentencer_T2 177-305 Sentence denotes The voltage-dependent anion channel (VDAC) is a pore-forming protein expressed in the outer membrane of eukaryotic mitochondria.
T2 177-305 Sentence denotes The voltage-dependent anion channel (VDAC) is a pore-forming protein expressed in the outer membrane of eukaryotic mitochondria.
TextSentencer_T3 306-487 Sentence denotes Three isoforms of it, i.e., VDAC1, VDAC2, and VDAC3, are known to be expressed in mammals; however, the question as to which is the main isoform in mitochondria is still unanswered.
T3 306-487 Sentence denotes Three isoforms of it, i.e., VDAC1, VDAC2, and VDAC3, are known to be expressed in mammals; however, the question as to which is the main isoform in mitochondria is still unanswered.
TextSentencer_T4 488-671 Sentence denotes To address this question, we first prepared standard VDACs by using a bacterial expression system and raised various antibodies against them by using synthetic peptides as immunogens.
T4 488-671 Sentence denotes To address this question, we first prepared standard VDACs by using a bacterial expression system and raised various antibodies against them by using synthetic peptides as immunogens.
TextSentencer_T5 672-906 Sentence denotes Of the three bacterially expressed VDAC isoforms, VDAC3 showed faster migration in SDS-polyacrylamide gels than VDAC1 and VDAC2, although VDAC2 is longer than VDAC1 and VDAC3, due to a 12-amino acid extension of its N-terminal region.
T5 672-906 Sentence denotes Of the three bacterially expressed VDAC isoforms, VDAC3 showed faster migration in SDS-polyacrylamide gels than VDAC1 and VDAC2, although VDAC2 is longer than VDAC1 and VDAC3, due to a 12-amino acid extension of its N-terminal region.
TextSentencer_T6 907-1120 Sentence denotes Even with careful structural characterization of the expressed VDACs by LC-MS/MS analysis, serious structural modifications of VDACs causing changes in their migration in SDS-polyacrylamide gels were not detected.
T6 907-1120 Sentence denotes Even with careful structural characterization of the expressed VDACs by LC-MS/MS analysis, serious structural modifications of VDACs causing changes in their migration in SDS-polyacrylamide gels were not detected.
TextSentencer_T7 1121-1235 Sentence denotes Next, immunoreactivities of the raised antibodies toward these bacterially expressed VDAC isoforms were evaluated.
T7 1121-1235 Sentence denotes Next, immunoreactivities of the raised antibodies toward these bacterially expressed VDAC isoforms were evaluated.
TextSentencer_T8 1236-1365 Sentence denotes Trials to prepare specific antibodies against the three individual VDAC isoforms were not successful except in the case of VDAC1.
T8 1236-1365 Sentence denotes Trials to prepare specific antibodies against the three individual VDAC isoforms were not successful except in the case of VDAC1.
TextSentencer_T9 1366-1581 Sentence denotes However, using a synthetic peptide corresponding to the highly conserved region among the three VDACs, we were successful in preparing an antibody showing essentially equal immunoreactivities toward all three VDACs.
T9 1366-1581 Sentence denotes However, using a synthetic peptide corresponding to the highly conserved region among the three VDACs, we were successful in preparing an antibody showing essentially equal immunoreactivities toward all three VDACs.
TextSentencer_T10 1582-1790 Sentence denotes When mitochondrial outer membrane proteins of various rat tissues were subjected to 2-dimensional electrophoresis followed by immunoblotting with this antibody, six immunoreactive protein spots were detected.
T10 1582-1790 Sentence denotes When mitochondrial outer membrane proteins of various rat tissues were subjected to 2-dimensional electrophoresis followed by immunoblotting with this antibody, six immunoreactive protein spots were detected.
TextSentencer_T11 1791-1901 Sentence denotes These spots were characterized by LC-MS/MS analysis, and the signal intensities among the spots were compared.
T11 1791-1901 Sentence denotes These spots were characterized by LC-MS/MS analysis, and the signal intensities among the spots were compared.
TextSentencer_T12 1902-2103 Sentence denotes As a result, the signal intensity of the spot representing VDAC1 was the highest, and thus, VDAC1 was concluded to be the most abundantly expressed of the three VDAC isoforms in mammalian mitochondria.
T12 1902-2103 Sentence denotes As a result, the signal intensity of the spot representing VDAC1 was the highest, and thus, VDAC1 was concluded to be the most abundantly expressed of the three VDAC isoforms in mammalian mitochondria.