> top > projects > sentences > docs > PubMed:17111384 > annotations
sentences Home  

PubMed:17111384 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-129 Sentence denotes Heterogeneity of aldehyde dehydrogenase expression in lung cancer cell lines is revealed by Aldefluor flow cytometry-based assay.
T1 0-129 Sentence denotes Heterogeneity of aldehyde dehydrogenase expression in lung cancer cell lines is revealed by Aldefluor flow cytometry-based assay.
TextSentencer_T2 130-141 Sentence denotes BACKGROUND:
T2 130-141 Sentence denotes BACKGROUND:
TextSentencer_T3 142-249 Sentence denotes We have been interested in studying the roles of two aldehyde dehydrogenases in the biology of lung cancer.
T3 142-249 Sentence denotes We have been interested in studying the roles of two aldehyde dehydrogenases in the biology of lung cancer.
TextSentencer_T4 250-494 Sentence denotes In this study, we seek to apply Aldefluor flow cytometry-based assay for the measurement of aldehyde dehydrogenase (ALDH) activity in lung cancer cell lines, which may become a new tool that will facilitate our continued research in this field.
T4 250-494 Sentence denotes In this study, we seek to apply Aldefluor flow cytometry-based assay for the measurement of aldehyde dehydrogenase (ALDH) activity in lung cancer cell lines, which may become a new tool that will facilitate our continued research in this field.
TextSentencer_T5 495-515 Sentence denotes EXPERIMENTAL DESIGN:
T5 495-515 Sentence denotes EXPERIMENTAL DESIGN:
TextSentencer_T6 516-663 Sentence denotes Several established lung cancer cell lines were used, including A549 cell line expressing siRNA against aldehyde dehydrogenase class-1A1 (ALDH1A1).
T6 516-663 Sentence denotes Several established lung cancer cell lines were used, including A549 cell line expressing siRNA against aldehyde dehydrogenase class-1A1 (ALDH1A1).
TextSentencer_T7 664-803 Sentence denotes Western blot analysis, spectrophotometry assay, and Aldefluor staining were used to measure protein or enzyme activity in these cell lines.
T7 664-803 Sentence denotes Western blot analysis, spectrophotometry assay, and Aldefluor staining were used to measure protein or enzyme activity in these cell lines.
TextSentencer_T8 804-1017 Sentence denotes For the purpose of measurement of ALDH activity by Aldefluor in cells with known high ALDH levels, cells were mixed 1:10 with immortalized lung epithelial cell line (Beas-2B), which is known to lack ALDH activity.
T8 804-1017 Sentence denotes For the purpose of measurement of ALDH activity by Aldefluor in cells with known high ALDH levels, cells were mixed 1:10 with immortalized lung epithelial cell line (Beas-2B), which is known to lack ALDH activity.
TextSentencer_T9 1018-1110 Sentence denotes To delineate dead cells, double staining using Aldefluor and propidium iodide (PI) was done.
T9 1018-1110 Sentence denotes To delineate dead cells, double staining using Aldefluor and propidium iodide (PI) was done.
TextSentencer_T10 1111-1262 Sentence denotes Double staining was also used to detect changes in ALDH activity in two different cell lines after treatment with 4-hydroperoxycyclophosphamide (4-HC).
T10 1111-1262 Sentence denotes Double staining was also used to detect changes in ALDH activity in two different cell lines after treatment with 4-hydroperoxycyclophosphamide (4-HC).
TextSentencer_T11 1263-1271 Sentence denotes RESULTS:
T11 1263-1271 Sentence denotes RESULTS:
TextSentencer_T12 1272-1375 Sentence denotes Our results show a very good correlation between Aldefluor, Western blot, and spectrophotometry assays.
T12 1272-1375 Sentence denotes Our results show a very good correlation between Aldefluor, Western blot, and spectrophotometry assays.
TextSentencer_T13 1376-1620 Sentence denotes Mixing experiments with Beas-2B cells allowed accurate assessment of ALDH activity in A549 cells at baseline and after siRNA expression, thus establishing an approach that facilitates the measurement of very high ALDH using the Aldefluor assay.
T13 1376-1620 Sentence denotes Mixing experiments with Beas-2B cells allowed accurate assessment of ALDH activity in A549 cells at baseline and after siRNA expression, thus establishing an approach that facilitates the measurement of very high ALDH using the Aldefluor assay.
TextSentencer_T14 1621-1807 Sentence denotes Aldefluor staining was able to detect heterogeneity in ALDH expression among as well as within the same cell lines and better assess viability after 4-HC treatment when combined with PI.
T14 1621-1807 Sentence denotes Aldefluor staining was able to detect heterogeneity in ALDH expression among as well as within the same cell lines and better assess viability after 4-HC treatment when combined with PI.
TextSentencer_T15 1808-1820 Sentence denotes CONCLUSIONS:
T15 1808-1820 Sentence denotes CONCLUSIONS:
TextSentencer_T16 1821-2037 Sentence denotes Aldefluor assay can be adapted successfully to measure ALDH activity in lung cancer cells and may have the advantage of providing real time changes in ALDH activity in viable cells treated with siRNA or chemotherapy.
T16 1821-2037 Sentence denotes Aldefluor assay can be adapted successfully to measure ALDH activity in lung cancer cells and may have the advantage of providing real time changes in ALDH activity in viable cells treated with siRNA or chemotherapy.