| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-88 |
Sentence |
denotes |
Membrane cholesterol content modulates ClC-2 gating and sensitivity to oxidative stress. |
| T2 |
89-174 |
Sentence |
denotes |
ClC-2 is a broadly expressed member of the voltage-gated ClC chloride channel family. |
| T3 |
175-365 |
Sentence |
denotes |
In this study, we aimed to evaluate the role of the membrane lipid environment in ClC-2 function, and in particular the effect of cholesterol and ClC-2 distribution in membrane microdomains. |
| T4 |
366-515 |
Sentence |
denotes |
Detergent-resistant and detergent-soluble microdomains (DSM) were isolated from stably transfected HEK293 cells by a discontinuous OptiPrep gradient. |
| T5 |
516-676 |
Sentence |
denotes |
ClC-2 was found concentrated in detergent-insoluble membranes in basal conditions and relocalized to DSM upon cholesterol depletion by methyl-beta-cyclodextrin. |
| T6 |
677-805 |
Sentence |
denotes |
As assessed by patch clamp recordings, relocalization was accompanied by acceleration of the activation kinetics of the channel. |
| T7 |
806-956 |
Sentence |
denotes |
A similar distribution and activation pattern were obtained when cells were treated with the oxidant tert-butyl hydroperoxide and after ATP depletion. |
| T8 |
957-1031 |
Sentence |
denotes |
In both cases activation was prevented by cholesterol enrichment of cells. |
| T9 |
1032-1261 |
Sentence |
denotes |
We conclude that the cholesterol environment regulates ClC-2 activity, and we provide evidence that the increase in ClC-2 activity in response to acute oxidative or metabolic stress involves relocalization of this channel to DSM. |
