| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-62 |
Sentence |
denotes |
Chick cartilage chondroitin sulfate proteoglycan core protein. |
| T1 |
0-62 |
Sentence |
denotes |
Chick cartilage chondroitin sulfate proteoglycan core protein. |
| T2 |
63-66 |
Sentence |
denotes |
II. |
| T2 |
63-66 |
Sentence |
denotes |
II. |
| T3 |
67-139 |
Sentence |
denotes |
Nucleotide sequence of cDNA clone and localization of the S103L epitope. |
| T3 |
67-139 |
Sentence |
denotes |
Nucleotide sequence of cDNA clone and localization of the S103L epitope. |
| T4 |
140-368 |
Sentence |
denotes |
A lambda gt11 expression library containing cDNA from total chick embryo was screened with S103L, a rat monoclonal antibody which reacts specifically with the core protein of the chick cartilage chondroitin sulfate proteoglycan. |
| T4 |
140-368 |
Sentence |
denotes |
A lambda gt11 expression library containing cDNA from total chick embryo was screened with S103L, a rat monoclonal antibody which reacts specifically with the core protein of the chick cartilage chondroitin sulfate proteoglycan. |
| T5 |
369-467 |
Sentence |
denotes |
One clone was identified which produced a 220-kDa beta-galactosidase/S103L-binding fusion protein. |
| T5 |
369-467 |
Sentence |
denotes |
One clone was identified which produced a 220-kDa beta-galactosidase/S103L-binding fusion protein. |
| T6 |
468-662 |
Sentence |
denotes |
Sequencing the entire 1.5-kilobase cDNA insert showed that it contained a single open reading frame, which encoded a portion of the proteoglycan core protein from the chondroitin sulfate domain. |
| T6 |
468-662 |
Sentence |
denotes |
Sequencing the entire 1.5-kilobase cDNA insert showed that it contained a single open reading frame, which encoded a portion of the proteoglycan core protein from the chondroitin sulfate domain. |
| T7 |
663-830 |
Sentence |
denotes |
This was confirmed by comparison with amino acid sequence data from peptide CS-B, which was derived from the chondroitin sulfate domain (Krueger, R.C., Jr., Fields, T. |
| T7 |
663-830 |
Sentence |
denotes |
This was confirmed by comparison with amino acid sequence data from peptide CS-B, which was derived from the chondroitin sulfate domain (Krueger, R.C., Jr., Fields, T. |
| T8 |
831-881 |
Sentence |
denotes |
A., Hildreth, J., IV, and Schwartz, N.B. (1990) J. |
| T8 |
831-881 |
Sentence |
denotes |
A., Hildreth, J., IV, and Schwartz, N.B. (1990) J. |
| T9 |
882-887 |
Sentence |
denotes |
Biol. |
| T9 |
882-887 |
Sentence |
denotes |
Biol. |
| T10 |
888-893 |
Sentence |
denotes |
Chem. |
| T10 |
888-893 |
Sentence |
denotes |
Chem. |
| T11 |
894-912 |
Sentence |
denotes |
265, 12075-12087). |
| T11 |
894-912 |
Sentence |
denotes |
265, 12075-12087). |
| T12 |
913-1083 |
Sentence |
denotes |
Furthermore, the 3' end of the insert overlapped with 23 bases at the 5' end of the published sequence for the C-terminal globular domain (Sai, S., Tanaka, T., Kosher, R. |
| T12 |
913-1083 |
Sentence |
denotes |
Furthermore, the 3' end of the insert overlapped with 23 bases at the 5' end of the published sequence for the C-terminal globular domain (Sai, S., Tanaka, T., Kosher, R. |
| T13 |
1084-1102 |
Sentence |
denotes |
A., and Tanzer, M. |
| T13 |
1084-1102 |
Sentence |
denotes |
A., and Tanzer, M. |
| T14 |
1103-1118 |
Sentence |
denotes |
L. (1986) Proc. |
| T14 |
1103-1118 |
Sentence |
denotes |
L. (1986) Proc. |
| T15 |
1119-1124 |
Sentence |
denotes |
Natl. |
| T15 |
1119-1124 |
Sentence |
denotes |
Natl. |
| T16 |
1125-1130 |
Sentence |
denotes |
Acad. |
| T16 |
1125-1130 |
Sentence |
denotes |
Acad. |
| T17 |
1131-1135 |
Sentence |
denotes |
Sci. |
| T17 |
1131-1135 |
Sentence |
denotes |
Sci. |
| T18 |
1136-1138 |
Sentence |
denotes |
U. |
| T18 |
1136-1138 |
Sentence |
denotes |
U. |
| T19 |
1139-1141 |
Sentence |
denotes |
S. |
| T19 |
1139-1141 |
Sentence |
denotes |
S. |
| T20 |
1142-1144 |
Sentence |
denotes |
A. |
| T20 |
1142-1144 |
Sentence |
denotes |
A. |
| T21 |
1145-1238 |
Sentence |
denotes |
83, 5081-5085), which oriented this clone, as well as the CS peptide, along the protein core. |
| T21 |
1145-1238 |
Sentence |
denotes |
83, 5081-5085), which oriented this clone, as well as the CS peptide, along the protein core. |
| T22 |
1239-1465 |
Sentence |
denotes |
The cDNA insert hybridized with a 9-kilobase mRNA from sternal chondrocytes as well as a similar sized message in brain but did not hybridize to any message from rat chondrosarcoma or from undifferentiated limb bud mesenchyme. |
| T22 |
1239-1465 |
Sentence |
denotes |
The cDNA insert hybridized with a 9-kilobase mRNA from sternal chondrocytes as well as a similar sized message in brain but did not hybridize to any message from rat chondrosarcoma or from undifferentiated limb bud mesenchyme. |
| T23 |
1466-1709 |
Sentence |
denotes |
In further studies, the fusion protein as well as a cyanogen bromide fragment (70 kDa) derived from it were isolated and shown to react with S103L, indicating that cleavage at methionine residues does not disrupt the antibody recognition site. |
| T23 |
1466-1709 |
Sentence |
denotes |
In further studies, the fusion protein as well as a cyanogen bromide fragment (70 kDa) derived from it were isolated and shown to react with S103L, indicating that cleavage at methionine residues does not disrupt the antibody recognition site. |
| T24 |
1710-1940 |
Sentence |
denotes |
Purification and N-terminal sequencing of the antigenic CNBr fragment derived from the fusion protein revealed that its N terminus is preceded by a methionine in the fusion protein and overlaps with the N terminus of peptide CS-B. |
| T24 |
1710-1940 |
Sentence |
denotes |
Purification and N-terminal sequencing of the antigenic CNBr fragment derived from the fusion protein revealed that its N terminus is preceded by a methionine in the fusion protein and overlaps with the N terminus of peptide CS-B. |
| T25 |
1941-2283 |
Sentence |
denotes |
As peptide CS-B is not recognized by S103L and the C terminus of peptide CS-B lies beyond the proteoglycan portion of the antigenic CNBr fragment, the S103L epitope is either contained within the 11 amino acids preceding the N terminus of peptide CS-B or it spans the clostripain cleavage site at the origin of the N terminus of peptide CS-B. |
| T25 |
1941-2283 |
Sentence |
denotes |
As peptide CS-B is not recognized by S103L and the C terminus of peptide CS-B lies beyond the proteoglycan portion of the antigenic CNBr fragment, the S103L epitope is either contained within the 11 amino acids preceding the N terminus of peptide CS-B or it spans the clostripain cleavage site at the origin of the N terminus of peptide CS-B. |