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PubMed:16807236 JSONTXT 35 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-40 Sentence denotes CCL5-CCR5-mediated apoptosis in T cells:
T2 41-104 Sentence denotes Requirement for glycosaminoglycan binding and CCL5 aggregation.
T3 105-256 Sentence denotes CCL5 (RANTES (regulated on activation normal T cell expressed and secreted)) and its cognate receptor, CCR5, have been implicated in T cell activation.
T4 257-423 Sentence denotes CCL5 binding to glycosaminoglycans (GAGs) on the cell surface or in extracellular matrix sequesters CCL5, thereby immobilizing CCL5 to provide the directional signal.
T5 424-590 Sentence denotes In two CCR5-expressing human T cell lines, PM1.CCR5 and MOLT4.CCR5, and in human peripheral blood-derived T cells, micromolar concentrations of CCL5 induce apoptosis.
T6 591-747 Sentence denotes CCL5-induced cell death involves the cytosolic release of cytochrome c, the activation of caspase-9 and caspase-3, and poly(ADP-ribose) polymerase cleavage.
T7 748-888 Sentence denotes CCL5-induced apoptosis is CCR5-dependent, since native PM1 and MOLT4 cells lacking CCR5 expression are resistant to CCL5-induced cell death.
T8 889-1077 Sentence denotes Furthermore, we implicate tyrosine 339 as a critical residue involved in CCL5-induced apoptosis, since PM1 cells expressing a tyrosine mutant receptor, CCR5Y339F, do not undergo apoptosis.
T9 1078-1161 Sentence denotes We show that CCL5-CCR5-mediated apoptosis is dependent on cell surface GAG binding.
T10 1162-1289 Sentence denotes The addition of exogenous heparin and chondroitin sulfate and GAG digestion from the cell surface protect cells from apoptosis.
T11 1290-1372 Sentence denotes Moreover, the non-GAG binding variant, (44AANA47)-CCL5, fails to induce apoptosis.
T12 1373-1564 Sentence denotes To address the role of aggregation in CCL5-mediated apoptosis, nonaggregating CCL5 mutant E66S, which forms dimers, and E26A, which form tetramers at micromolar concentrations, were utilized.
T13 1565-1737 Sentence denotes Unlike native CCL5, the E66S mutant fails to induce apoptosis, suggesting that tetramers are the minimal higher ordered CCL5 aggregates required for CCL5-induced apoptosis.
T14 1738-1918 Sentence denotes Viewed altogether, these data suggest that CCL5-GAG binding and CCL5 aggregation are important for CCL5 activity in T cells, specifically in the context of CCR5-mediated apoptosis.