| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-105 |
Sentence |
denotes |
Crystal structure of heparinase II from Pedobacter heparinus and its complex with a disaccharide product. |
| T2 |
106-273 |
Sentence |
denotes |
Heparinase II depolymerizes heparin and heparan sulfate glycosaminoglycans, yielding unsaturated oligosaccharide products through an elimination degradation mechanism. |
| T3 |
274-439 |
Sentence |
denotes |
This enzyme cleaves the oligosaccharide chain on the nonreducing end of either glucuronic or iduronic acid, sharing this characteristic with a chondroitin ABC lyase. |
| T4 |
440-725 |
Sentence |
denotes |
We have determined the first structure of a heparin-degrading lyase, that of heparinase II from Pedobacter heparinus (formerly Flavobacterium heparinum), in a ligand-free state at 2.15 A resolution and in complex with a disaccharide product of heparin degradation at 2.30 A resolution. |
| T5 |
726-902 |
Sentence |
denotes |
The protein is composed of three domains: an N-terminal alpha-helical domain, a central two-layered beta-sheet domain, and a C-terminal domain forming a two-layered beta-sheet. |
| T6 |
903-1049 |
Sentence |
denotes |
Heparinase II shows overall structural similarities to the polysaccharide lyase family 8 (PL8) enzymes chondroitin AC lyase and hyaluronate lyase. |
| T7 |
1050-1189 |
Sentence |
denotes |
In contrast to PL8 enzymes, however, heparinase II forms stable dimers, with the two active sites formed independently within each monomer. |
| T8 |
1190-1309 |
Sentence |
denotes |
The structure of the N-terminal domain of heparinase II is also similar to that of alginate lyases from the PL5 family. |
| T9 |
1310-1473 |
Sentence |
denotes |
A Zn2+ ion is bound within the central domain and plays an essential structural role in the stabilization of a loop forming one wall of the substrate-binding site. |
| T10 |
1474-1610 |
Sentence |
denotes |
The disaccharide binds in a long, deep canyon formed at the top of the N-terminal domain and by loops extending from the central domain. |
| T11 |
1611-1801 |
Sentence |
denotes |
Based on structural comparison with the lyases from the PL5 and PL8 families having bound substrates or products, the disaccharide found in heparinase II occupies the "+1" and "+2" subsites. |
| T12 |
1802-2001 |
Sentence |
denotes |
The structure of the enzyme-product complex, combined with data from previously characterized mutations, allows us to propose a putative chemical mechanism of heparin and heparan-sulfate degradation. |
