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PubMed:16512681 JSONTXT 40 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-117 Sentence denotes Comparative study of three proteomic quantitative methods, DIGE, cICAT, and iTRAQ, using 2D gel- or LC-MALDI TOF/TOF.
T1 0-117 Sentence denotes Comparative study of three proteomic quantitative methods, DIGE, cICAT, and iTRAQ, using 2D gel- or LC-MALDI TOF/TOF.
TextSentencer_T2 118-375 Sentence denotes A comparative study on the three quantitative methods frequently used in proteomics, 2D DIGE (difference gel electrophoresis), cICAT (cleavable isotope-coded affinity tags) and iTRAQ (isobaric tags for relative and absolute quantification), was carried out.
T2 118-375 Sentence denotes A comparative study on the three quantitative methods frequently used in proteomics, 2D DIGE (difference gel electrophoresis), cICAT (cleavable isotope-coded affinity tags) and iTRAQ (isobaric tags for relative and absolute quantification), was carried out.
TextSentencer_T3 376-555 Sentence denotes DIGE and cICAT are familiar techniques used in gel- and LC-based quantitative proteomics, respectively. iTRAQ is a new LC-based technique which is gradually gaining in popularity.
T3 376-555 Sentence denotes DIGE and cICAT are familiar techniques used in gel- and LC-based quantitative proteomics, respectively. iTRAQ is a new LC-based technique which is gradually gaining in popularity.
TextSentencer_T4 556-635 Sentence denotes A systematic comparison among these quantitative methods has not been reported.
T4 556-635 Sentence denotes A systematic comparison among these quantitative methods has not been reported.
TextSentencer_T5 636-863 Sentence denotes In this study, we conducted well-designed comparisons using a six-protein mixture, a reconstituted protein mixture (BSA spiked into human plasma devoid of six abundant proteins), and complex HCT-116 cell lysates as the samples.
T5 636-863 Sentence denotes In this study, we conducted well-designed comparisons using a six-protein mixture, a reconstituted protein mixture (BSA spiked into human plasma devoid of six abundant proteins), and complex HCT-116 cell lysates as the samples.
TextSentencer_T6 864-1006 Sentence denotes All three techniques yielded quantitative results with reasonable accuracy when the six-protein or the reconstituted protein mixture was used.
T6 864-1006 Sentence denotes All three techniques yielded quantitative results with reasonable accuracy when the six-protein or the reconstituted protein mixture was used.
TextSentencer_T7 1007-1120 Sentence denotes In DIGE, accurate quantification was sometimes compromised due to comigration or partial comigration of proteins.
T7 1007-1120 Sentence denotes In DIGE, accurate quantification was sometimes compromised due to comigration or partial comigration of proteins.
TextSentencer_T8 1121-1256 Sentence denotes The iTRAQ method is more susceptible to errors in precursor ion isolation, which could be manifested with increasing sample complexity.
T8 1121-1256 Sentence denotes The iTRAQ method is more susceptible to errors in precursor ion isolation, which could be manifested with increasing sample complexity.
TextSentencer_T9 1257-1369 Sentence denotes The quantification sensitivity of each method was estimated by the number of peptides detected for each protein.
T9 1257-1369 Sentence denotes The quantification sensitivity of each method was estimated by the number of peptides detected for each protein.
TextSentencer_T10 1370-1563 Sentence denotes In this regard, the global-tagging iTRAQ technique was more sensitive than the cysteine-specific cICAT method, which in turn was as sensitive as, if not more sensitive than, the DIGE technique.
T10 1370-1563 Sentence denotes In this regard, the global-tagging iTRAQ technique was more sensitive than the cysteine-specific cICAT method, which in turn was as sensitive as, if not more sensitive than, the DIGE technique.
TextSentencer_T11 1564-1757 Sentence denotes Protein profiling on HCT-116 and HCT-116 p53 -/- cell lysates displayed limited overlapping among proteins identified by the three methods, suggesting the complementary nature of these methods.
T11 1564-1757 Sentence denotes Protein profiling on HCT-116 and HCT-116 p53 -/- cell lysates displayed limited overlapping among proteins identified by the three methods, suggesting the complementary nature of these methods.