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PubMed:16455656 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-90 Sentence denotes CpSufE activates the cysteine desulfurase CpNifS for chloroplastic Fe-S cluster formation.
T2 91-310 Sentence denotes CpNifS, a cysteine desulfurase required to supply sulfur for ironsulfur cluster biogenesis in Arabidopsis thaliana chloroplasts, belongs to a class of NifS-like enzymes with low endogenous cysteine desulfurase activity.
T3 311-362 Sentence denotes Its bacterial homologue SufS is stimulated by SufE.
T4 363-527 Sentence denotes Here we characterize the Arabidopsis chloroplast protein CpSufE, which has an N-terminal SufE-like domain and a C-terminal BolA-like domain unique to higher plants.
T5 528-653 Sentence denotes CpSufE is targeted to the chloroplast stroma, indicated by green fluorescent protein localization and immunoblot experiments.
T6 654-747 Sentence denotes Like CpNifS, CpSufE is expressed in all major tissues, with higher expression in green parts.
T7 748-814 Sentence denotes Its expression is light-dependent and regulated at the mRNA level.
T8 815-997 Sentence denotes The addition of purified recombinant CpSufE increased the Vmax for the cysteine desulfurase activity of CpNifS over 40-fold and decreased the KM toward cysteine from 0.1 to 0.043 mm.
T9 998-1203 Sentence denotes In contrast, CpSufE addition decreased the affinity of CpNifS for selenocysteine, as indicated by an increase in the KM from 2.9 to 4.17 mm, and decreased the Vmax for selenocysteine lyase activity by 30%.
T10 1204-1330 Sentence denotes CpSufE forms dynamic complexes with CpNifS, indicated by gel filtration, native PAGE, and affinity chromatography experiments.
T11 1331-1478 Sentence denotes A mutant of CpSufE in which the single cysteine was changed to serine was not active in stimulating CpNifS, although it did compete with WT CpSufE.
T12 1479-1622 Sentence denotes The iron-sulfur cluster reconstitution activity of the CpNifS-CpSufE complex toward apoferredoxin was 20-fold higher than that of CpNifS alone.
T13 1623-1754 Sentence denotes We conclude that CpNifS and CpSufE together form a cysteine desulfurase required for iron-sulfur cluster formation in chloroplasts.