| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-145 |
Sentence |
denotes |
A specific detection of GlcNAcbeta1-6Manalpha1 branches in N-linked glycoproteins based on the specificity of N-acetylglucosaminyltransferase VI. |
| T1 |
0-145 |
Sentence |
denotes |
A specific detection of GlcNAcbeta1-6Manalpha1 branches in N-linked glycoproteins based on the specificity of N-acetylglucosaminyltransferase VI. |
| T1 |
0-145 |
Sentence |
denotes |
A specific detection of GlcNAcbeta1-6Manalpha1 branches in N-linked glycoproteins based on the specificity of N-acetylglucosaminyltransferase VI. |
| TextSentencer_T2 |
146-340 |
Sentence |
denotes |
Malignant transformation is often accompanied by an aberrant glycosylation profile of the cell surface-in particular, the production of GlcNAcbeta1-6Manalpha1 branches in N-linked glycoproteins. |
| T2 |
146-340 |
Sentence |
denotes |
Malignant transformation is often accompanied by an aberrant glycosylation profile of the cell surface-in particular, the production of GlcNAcbeta1-6Manalpha1 branches in N-linked glycoproteins. |
| T2 |
146-340 |
Sentence |
denotes |
Malignant transformation is often accompanied by an aberrant glycosylation profile of the cell surface-in particular, the production of GlcNAcbeta1-6Manalpha1 branches in N-linked glycoproteins. |
| TextSentencer_T3 |
341-515 |
Sentence |
denotes |
To identify the target glycoproteins, we show a method using recombinant chicken N-acetylglucosaminyltransferase VI (GnT VI) and radiolabeled uridine (5'-)diphosphate-GlcNAc. |
| T3 |
341-515 |
Sentence |
denotes |
To identify the target glycoproteins, we show a method using recombinant chicken N-acetylglucosaminyltransferase VI (GnT VI) and radiolabeled uridine (5'-)diphosphate-GlcNAc. |
| T3 |
341-515 |
Sentence |
denotes |
To identify the target glycoproteins, we show a method using recombinant chicken N-acetylglucosaminyltransferase VI (GnT VI) and radiolabeled uridine (5'-)diphosphate-GlcNAc. |
| TextSentencer_T4 |
516-707 |
Sentence |
denotes |
The assay exploits the fact that GnT VI has a strict requirement for the GlcNAcbeta1-6Manalpha1 structure for activity, when a pyridylaminated free N-glycan is used as the acceptor substrate. |
| T4 |
516-707 |
Sentence |
denotes |
The assay exploits the fact that GnT VI has a strict requirement for the GlcNAcbeta1-6Manalpha1 structure for activity, when a pyridylaminated free N-glycan is used as the acceptor substrate. |
| T4 |
516-707 |
Sentence |
denotes |
The assay exploits the fact that GnT VI has a strict requirement for the GlcNAcbeta1-6Manalpha1 structure for activity, when a pyridylaminated free N-glycan is used as the acceptor substrate. |
| TextSentencer_T5 |
708-1074 |
Sentence |
denotes |
Human asialo-agalacto alpha1-acid glycoprotein (AGP), which is known to contain GlcNAcbeta1-6Manalpha1 branches in its N-linked glycan chains, was radiolabeled when reacted with GnT VI, whereas human asialo-agalacto transferrin and bovine fetuin, neither of which contains a GlcNAcbeta1-6Manalpha1 structure were not, thus corroborating the specificity of the assay. |
| T5 |
708-1074 |
Sentence |
denotes |
Human asialo-agalacto alpha1-acid glycoprotein (AGP), which is known to contain GlcNAcbeta1-6Manalpha1 branches in its N-linked glycan chains, was radiolabeled when reacted with GnT VI, whereas human asialo-agalacto transferrin and bovine fetuin, neither of which contains a GlcNAcbeta1-6Manalpha1 structure were not, thus corroborating the specificity of the assay. |
| T5 |
708-1074 |
Sentence |
denotes |
Human asialo-agalacto alpha1-acid glycoprotein (AGP), which is known to contain GlcNAcbeta1-6Manalpha1 branches in its N-linked glycan chains, was radiolabeled when reacted with GnT VI, whereas human asialo-agalacto transferrin and bovine fetuin, neither of which contains a GlcNAcbeta1-6Manalpha1 structure were not, thus corroborating the specificity of the assay. |
| TextSentencer_T6 |
1075-1200 |
Sentence |
denotes |
Several proteins from human serum after pretreatment with sialidase and beta-galactosidase could be detected using the assay. |
| T6 |
1075-1200 |
Sentence |
denotes |
Several proteins from human serum after pretreatment with sialidase and beta-galactosidase could be detected using the assay. |
| T6 |
1075-1200 |
Sentence |
denotes |
Several proteins from human serum after pretreatment with sialidase and beta-galactosidase could be detected using the assay. |
| TextSentencer_T7 |
1201-1326 |
Sentence |
denotes |
One was identified as AGP from its mobility on SDS-PAGE, demonstrating the potential of this assay even with crude materials. |
| T7 |
1201-1326 |
Sentence |
denotes |
One was identified as AGP from its mobility on SDS-PAGE, demonstrating the potential of this assay even with crude materials. |
| T7 |
1201-1326 |
Sentence |
denotes |
One was identified as AGP from its mobility on SDS-PAGE, demonstrating the potential of this assay even with crude materials. |
| TextSentencer_T8 |
1327-1526 |
Sentence |
denotes |
Furthermore, this method could detect a protein that was also positively stained with leukoagglutinating phytohemagglutinin (L(4)-PHA) using glycoproteins prepared from WiDr human colon cancer cells. |
| T8 |
1327-1526 |
Sentence |
denotes |
Furthermore, this method could detect a protein that was also positively stained with leukoagglutinating phytohemagglutinin (L(4)-PHA) using glycoproteins prepared from WiDr human colon cancer cells. |
| T8 |
1327-1526 |
Sentence |
denotes |
Furthermore, this method could detect a protein that was also positively stained with leukoagglutinating phytohemagglutinin (L(4)-PHA) using glycoproteins prepared from WiDr human colon cancer cells. |
| TextSentencer_T9 |
1527-1641 |
Sentence |
denotes |
This method should provide a useful complement to the current method, which relies on the specificity of L(4)-PHA. |
| T9 |
1527-1641 |
Sentence |
denotes |
This method should provide a useful complement to the current method, which relies on the specificity of L(4)-PHA. |
| T9 |
1527-1641 |
Sentence |
denotes |
This method should provide a useful complement to the current method, which relies on the specificity of L(4)-PHA. |