| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-104 |
Sentence |
denotes |
Proteoglycan mechanics studied by single-molecule force spectroscopy of allotypic cell adhesion glycans. |
| T2 |
105-260 |
Sentence |
denotes |
Early Metazoans had to evolve the first cell adhesion system addressed to maintaining stable interactions between cells constituting different individuals. |
| T3 |
261-407 |
Sentence |
denotes |
As the oldest extant multicellular animals, sponges are good candidates to have remnants of the molecules responsible for that crucial innovation. |
| T4 |
408-613 |
Sentence |
denotes |
Sponge cells associate in a species-specific process through multivalent calcium-dependent interactions of carbohydrate structures on an extracellular membrane-bound proteoglycan termed aggregation factor. |
| T5 |
614-780 |
Sentence |
denotes |
Single-molecule force spectroscopy studies of the mechanics of aggregation factor self-binding indicate the existence of intermolecular carbohydrate adhesion domains. |
| T6 |
781-963 |
Sentence |
denotes |
A 200-kDa aggregation factor glycan (g200) involved in cell adhesion exhibits interindividual differences in size and epitope content which suggest the existence of allelic variants. |
| T7 |
964-1062 |
Sentence |
denotes |
We have purified two of these g200 distinct forms from two individuals of the same sponge species. |
| T8 |
1063-1155 |
Sentence |
denotes |
Comparison of allotypic versus isotypic g200 binding forces reveals significant differences. |
| T9 |
1156-1313 |
Sentence |
denotes |
Surface plasmon resonance measurements show that g200 self-adhesion is much stronger than its binding to other unrelated glycans such as chondroitin sulfate. |
| T10 |
1314-1505 |
Sentence |
denotes |
This adhesive specificity through multiple carbohydrate binding domains is a type of cooperative interaction that can contribute to explain some functions of modular proteoglycans in general. |
| T11 |
1506-1787 |
Sentence |
denotes |
From our results it can be deduced that the binding strength/surface area between two aggregation factor molecules is comparable with that of focal contacts in vertebrate cells, indicating that strong carbohydrate-based cell adhesions evolved at the very start of Metazoan history. |
