| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-84 |
Sentence |
denotes |
Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase. |
| T1 |
0-84 |
Sentence |
denotes |
Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase. |
| T1 |
0-84 |
Sentence |
denotes |
Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase. |
| TextSentencer_T2 |
85-367 |
Sentence |
denotes |
The synthesis of N-acetylneuraminate (Neu5Ac), the main form of sialic acid, proceeds in vertebrates through the condensation of N-acetylmannosamine 6-phosphate and phosphoenolpyruvate to Neu5Ac-9-phosphate, followed by the dephosphorylation of the latter by a specific phosphatase. |
| T2 |
85-367 |
Sentence |
denotes |
The synthesis of N-acetylneuraminate (Neu5Ac), the main form of sialic acid, proceeds in vertebrates through the condensation of N-acetylmannosamine 6-phosphate and phosphoenolpyruvate to Neu5Ac-9-phosphate, followed by the dephosphorylation of the latter by a specific phosphatase. |
| T2 |
85-367 |
Sentence |
denotes |
The synthesis of N-acetylneuraminate (Neu5Ac), the main form of sialic acid, proceeds in vertebrates through the condensation of N-acetylmannosamine 6-phosphate and phosphoenolpyruvate to Neu5Ac-9-phosphate, followed by the dephosphorylation of the latter by a specific phosphatase. |
| TextSentencer_T3 |
368-441 |
Sentence |
denotes |
The sequence encoding Neu5Ac-9-phosphate phosphatase (Neu5Ac-9-Pase; E.C. |
| T3 |
368-441 |
Sentence |
denotes |
The sequence encoding Neu5Ac-9-phosphate phosphatase (Neu5Ac-9-Pase; E.C. |
| T3 |
368-486 |
Sentence |
denotes |
The sequence encoding Neu5Ac-9-phosphate phosphatase (Neu5Ac-9-Pase; E.C. 3.1.3.29) has not been determined until now. |
| TextSentencer_T4 |
442-486 |
Sentence |
denotes |
3.1.3.29) has not been determined until now. |
| T4 |
442-486 |
Sentence |
denotes |
3.1.3.29) has not been determined until now. |
| TextSentencer_T5 |
487-567 |
Sentence |
denotes |
In this work, we have purified Neu5Ac-9-Pase more than 1000-fold from rat liver. |
| T4 |
487-567 |
Sentence |
denotes |
In this work, we have purified Neu5Ac-9-Pase more than 1000-fold from rat liver. |
| T5 |
487-567 |
Sentence |
denotes |
In this work, we have purified Neu5Ac-9-Pase more than 1000-fold from rat liver. |
| TextSentencer_T6 |
568-726 |
Sentence |
denotes |
Its dependency on Mg2+ and the fact that it was inhibited by vanadate and Ca2+ suggested that it belonged to the haloacid dehalogenase family of phosphatases. |
| T5 |
568-726 |
Sentence |
denotes |
Its dependency on Mg2+ and the fact that it was inhibited by vanadate and Ca2+ suggested that it belonged to the haloacid dehalogenase family of phosphatases. |
| T6 |
568-726 |
Sentence |
denotes |
Its dependency on Mg2+ and the fact that it was inhibited by vanadate and Ca2+ suggested that it belonged to the haloacid dehalogenase family of phosphatases. |
| TextSentencer_T7 |
727-1070 |
Sentence |
denotes |
Trypsin digestion and mass spectrometry analysis of a polypeptide of about 30 kDa that co-eluted with the activity in the last purification step indicated the presence of a protein designated "haloacid dehalogenase-like hydrolase domain containing 4." The human ortholog of this protein is encoded by a 2-exon gene present on chromosome 20p11. |
| T6 |
727-1070 |
Sentence |
denotes |
Trypsin digestion and mass spectrometry analysis of a polypeptide of about 30 kDa that co-eluted with the activity in the last purification step indicated the presence of a protein designated "haloacid dehalogenase-like hydrolase domain containing 4." The human ortholog of this protein is encoded by a 2-exon gene present on chromosome 20p11. |
| T7 |
727-1070 |
Sentence |
denotes |
Trypsin digestion and mass spectrometry analysis of a polypeptide of about 30 kDa that co-eluted with the activity in the last purification step indicated the presence of a protein designated "haloacid dehalogenase-like hydrolase domain containing 4." The human ortholog of this protein is encoded by a 2-exon gene present on chromosome 20p11. |
| TextSentencer_T8 |
1071-1194 |
Sentence |
denotes |
The human protein was overexpressed in Escherichia coli as a fusion protein with a polyHis tag and purified to homogeneity. |
| T7 |
1071-1194 |
Sentence |
denotes |
The human protein was overexpressed in Escherichia coli as a fusion protein with a polyHis tag and purified to homogeneity. |
| T8 |
1071-1194 |
Sentence |
denotes |
The human protein was overexpressed in Escherichia coli as a fusion protein with a polyHis tag and purified to homogeneity. |
| TextSentencer_T9 |
1195-1324 |
Sentence |
denotes |
The recombinant enzyme displayed a >230-fold higher catalytic efficiency on Neu5Ac-9-phosphate than on its second best substrate. |
| T8 |
1195-1324 |
Sentence |
denotes |
The recombinant enzyme displayed a >230-fold higher catalytic efficiency on Neu5Ac-9-phosphate than on its second best substrate. |
| T9 |
1195-1324 |
Sentence |
denotes |
The recombinant enzyme displayed a >230-fold higher catalytic efficiency on Neu5Ac-9-phosphate than on its second best substrate. |
| TextSentencer_T10 |
1325-1400 |
Sentence |
denotes |
Its properties were similar to those of the enzyme purified from rat liver. |
| T9 |
1325-1400 |
Sentence |
denotes |
Its properties were similar to those of the enzyme purified from rat liver. |
| T10 |
1325-1400 |
Sentence |
denotes |
Its properties were similar to those of the enzyme purified from rat liver. |
| TextSentencer_T11 |
1401-1553 |
Sentence |
denotes |
Neu5Ac inhibited the enzymatic activity by 50% at 15 mM, indicating that no significant inhibition is exerted at physiological concentrations of Neu5Ac. |
| T10 |
1401-1553 |
Sentence |
denotes |
Neu5Ac inhibited the enzymatic activity by 50% at 15 mM, indicating that no significant inhibition is exerted at physiological concentrations of Neu5Ac. |
| T11 |
1401-1553 |
Sentence |
denotes |
Neu5Ac inhibited the enzymatic activity by 50% at 15 mM, indicating that no significant inhibition is exerted at physiological concentrations of Neu5Ac. |
| TextSentencer_T12 |
1554-1724 |
Sentence |
denotes |
The identification of the gene encoding Neu5Ac-9-Pase will facilitate studies aimed at testing its potential implication in unexplained forms of glycosylation deficiency. |
| T11 |
1554-1724 |
Sentence |
denotes |
The identification of the gene encoding Neu5Ac-9-Pase will facilitate studies aimed at testing its potential implication in unexplained forms of glycosylation deficiency. |
| T12 |
1554-1724 |
Sentence |
denotes |
The identification of the gene encoding Neu5Ac-9-Pase will facilitate studies aimed at testing its potential implication in unexplained forms of glycosylation deficiency. |