| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-83 |
Sentence |
denotes |
Isolation of a calmodulin-binding transcription factor from rice (Oryza sativa L.). |
| T2 |
84-204 |
Sentence |
denotes |
Calmodulin (CaM) regulates diverse cellular functions by modulating the activities of a variety of enzymes and proteins. |
| T3 |
205-291 |
Sentence |
denotes |
However, direct modulation of transcription factors by CaM has been poorly understood. |
| T4 |
292-443 |
Sentence |
denotes |
In this study, we isolated a putative transcription factor by screening a rice cDNA expression library by using CaM:horse-radish peroxidase as a probe. |
| T5 |
444-791 |
Sentence |
denotes |
This factor, which we have designated OsCBT (Oryza sativa CaM-binding transcription factor), has structural features similar to Arabidopsis AtSRs/AtCAMTAs and encodes a 103-kDa protein because it contains a CG-1 homology DNA-binding domain, three ankyrin repeats, a putative transcriptional activation domain, and five putative CaM-binding motifs. |
| T6 |
792-1002 |
Sentence |
denotes |
By using a gel overlay assay, gel mobility shift assays, and site-directed mutagenesis, we showed that OsCBT has two different types of functional CaM-binding domains, an IQ motif, and a Ca(2+)-dependent motif. |
| T7 |
1003-1105 |
Sentence |
denotes |
To determine the DNA binding specificity of OsCBT, we employed a random binding site selection method. |
| T8 |
1106-1253 |
Sentence |
denotes |
This analysis showed that OsCBT preferentially binds to the sequence 5'-TWCG(C/T)GTKKKKTKCG-3' (W and K represent A or C and T or G, respectively). |
| T9 |
1254-1454 |
Sentence |
denotes |
OsCBT was able to bind this sequence and activate beta-glucuronidase reporter gene expression driven by a minimal promoter containing tandem repeats of these sequences in Arabidopsis leaf protoplasts. |
| T10 |
1455-1617 |
Sentence |
denotes |
Green fluorescent protein fusions of two putative nuclear localization signals of OsCBT, a bipartite and a SV40 type, were predominantly localized in the nucleus. |
| T11 |
1618-1736 |
Sentence |
denotes |
Most interestingly, the transcriptional activation mediated by OsCBT was inhibited by co-transfection with a CaM gene. |
| T12 |
1737-1830 |
Sentence |
denotes |
Taken together, our results suggest that OsCBT is a transcription activator modulated by CaM. |
