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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-73 Sentence denotes Characterisation of a 1,4-beta-fucoside hydrolase degrading colanic acid.
T1 0-73 Sentence denotes Characterisation of a 1,4-beta-fucoside hydrolase degrading colanic acid.
TextSentencer_T2 74-244 Sentence denotes A novel colanic acid-degrading enzyme was isolated from a mixed culture filtrate obtained by enrichment culturing of a compost sample using colanic acid as carbon source.
T2 74-244 Sentence denotes A novel colanic acid-degrading enzyme was isolated from a mixed culture filtrate obtained by enrichment culturing of a compost sample using colanic acid as carbon source.
TextSentencer_T3 245-332 Sentence denotes The enzyme was partially purified resulting in a 17-fold increase in specific activity.
T3 245-332 Sentence denotes The enzyme was partially purified resulting in a 17-fold increase in specific activity.
TextSentencer_T4 333-487 Sentence denotes Further purification by Native PAGE revealed that the enzyme is part of a high-molecular weight multi protein complex of at least six individual proteins.
T4 333-487 Sentence denotes Further purification by Native PAGE revealed that the enzyme is part of a high-molecular weight multi protein complex of at least six individual proteins.
TextSentencer_T5 488-624 Sentence denotes The enzyme showed a temperature optimum at 50 degrees C while after 5h at 50 degrees C and pH7 still 70% of the total activity was left.
T5 488-624 Sentence denotes The enzyme showed a temperature optimum at 50 degrees C while after 5h at 50 degrees C and pH7 still 70% of the total activity was left.
TextSentencer_T6 625-660 Sentence denotes The pH optimum was found to be pH7.
T6 625-660 Sentence denotes The pH optimum was found to be pH7.
TextSentencer_T7 661-843 Sentence denotes Analysis of the degradation products showed that the enzyme is a novel 1,4-beta-fucoside hydrolase that liberates repeating units of colanic acid with varying degrees of acetylation.
T7 661-843 Sentence denotes Analysis of the degradation products showed that the enzyme is a novel 1,4-beta-fucoside hydrolase that liberates repeating units of colanic acid with varying degrees of acetylation.
TextSentencer_T8 844-968 Sentence denotes Km and Vmax of the enzyme were determined against the native substrate as well as its de-O-acetylated and depyruvated forms.
T8 844-968 Sentence denotes Km and Vmax of the enzyme were determined against the native substrate as well as its de-O-acetylated and depyruvated forms.
TextSentencer_T9 969-1072 Sentence denotes Compared to the native substrate the affinity of the enzyme for the modified substrates was much lower.
T9 969-1072 Sentence denotes Compared to the native substrate the affinity of the enzyme for the modified substrates was much lower.
TextSentencer_T10 1073-1170 Sentence denotes However, for the de-O-acetylated sample a dramatic increase in catalytic efficiency was observed.
T10 1073-1170 Sentence denotes However, for the de-O-acetylated sample a dramatic increase in catalytic efficiency was observed.
TextSentencer_T11 1171-1323 Sentence denotes The native form of the substrate showed substrate inhibition at high concentrations, probably due to the formation of nonproductive substrate complexes.
T11 1171-1323 Sentence denotes The native form of the substrate showed substrate inhibition at high concentrations, probably due to the formation of nonproductive substrate complexes.
TextSentencer_T12 1324-1426 Sentence denotes Removal of the acetyl groups probably prevents this effect resulting in a higher catalytic efficiency.
T12 1324-1426 Sentence denotes Removal of the acetyl groups probably prevents this effect resulting in a higher catalytic efficiency.