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PubMed:15788404 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-180 Sentence denotes Biochemical and molecular characterization of a novel choline-specific glycerophosphodiester phosphodiesterase belonging to the nucleotide pyrophosphatase/phosphodiesterase family.
T2 181-392 Sentence denotes Nucleotide pyrophosphatases/phosphodiesterases (NPPs) are ubiquitous membrane-associated or secreted ectoenzymes that release nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives.
T3 393-509 Sentence denotes The mammalian NPP family comprises seven members, but only three of these (NPP1-3) have been studied in some detail.
T4 510-659 Sentence denotes Previously we showed that lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) to produce lysophosphatidic acid, is identical to NPP2.
T5 660-770 Sentence denotes More recently an uncharacterized novel NPP member (NPP7) was shown to have alkaline sphingomyelinase activity.
T6 771-867 Sentence denotes These findings raised the possibility that other members of the NPP family act on phospholipids.
T7 868-990 Sentence denotes Here we show that the sixth member of the NPP family, NPP6, is a choline-specific glycerophosphodiester phosphodiesterase.
T8 991-1145 Sentence denotes The sequence of NPP6 encodes a transmembrane protein containing an NPP domain with significant homology to NPP4, NPP5, and NPP7/alkaline sphingomyelinase.
T9 1146-1296 Sentence denotes When expressed in HeLa cells, NPP6 was detected in both the cells and the cell culture medium as judged by Western blotting and by enzymatic activity.
T10 1297-1518 Sentence denotes Recombinant NPP6 efficiently hydrolyzed the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, but not the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate.
T11 1519-1676 Sentence denotes In addition, NPP6 hydrolyzed LPC to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity.
T12 1677-1784 Sentence denotes NPP6 showed a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids.
T13 1785-1873 Sentence denotes It also hydrolyzed glycerophosphorylcholine and sphingosylphosphorylcholine efficiently.
T14 1874-2024 Sentence denotes In mice, NPP6 mRNA was predominantly detected in kidney with a lesser expression in brain and heart, and in human it was detected in kidney and brain.
T15 2025-2203 Sentence denotes The present results suggest that NPP6 has a specific role through the hydrolysis of polyunsaturated LPC, glycerophosphorylcholine, or sphingosylphosphorylcholine in these organs.