> top > projects > sentences > docs > PubMed:15679724 > annotations
sentences Home  

PubMed:15679724 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-101 Sentence denotes cDNA cloning and immunologic characterization of a novel EF-1beta allergen from Penicillium citrinum.
T1 0-101 Sentence denotes cDNA cloning and immunologic characterization of a novel EF-1beta allergen from Penicillium citrinum.
TextSentencer_T2 102-113 Sentence denotes BACKGROUND:
T2 102-113 Sentence denotes BACKGROUND:
TextSentencer_T3 114-231 Sentence denotes We have identified previously that Penicillium citrinum is the most prevalent Penicillium species in the Taipei area.
T3 114-231 Sentence denotes We have identified previously that Penicillium citrinum is the most prevalent Penicillium species in the Taipei area.
TextSentencer_T4 232-339 Sentence denotes It is important to delineate the whole spectrum of allergenic components of this prevalent airborne fungus.
T4 232-339 Sentence denotes It is important to delineate the whole spectrum of allergenic components of this prevalent airborne fungus.
TextSentencer_T5 340-535 Sentence denotes The purpose of this study was to identify novel P. citrinum allergens through molecular cloning of allergen genes using a cDNA library of P. citrinum and sera from patients with bronchial asthma.
T5 340-535 Sentence denotes The purpose of this study was to identify novel P. citrinum allergens through molecular cloning of allergen genes using a cDNA library of P. citrinum and sera from patients with bronchial asthma.
TextSentencer_T6 536-544 Sentence denotes METHODS:
T6 536-544 Sentence denotes METHODS:
TextSentencer_T7 545-646 Sentence denotes A lambda-Uni-ZAP XR-based cDNA library of P. citrinum was screened with sera from asthmatic patients.
T7 545-646 Sentence denotes A lambda-Uni-ZAP XR-based cDNA library of P. citrinum was screened with sera from asthmatic patients.
TextSentencer_T8 647-735 Sentence denotes An IgE-binding cDNA clone was isolated and heterologously expressed in Escherichia coli.
T8 647-735 Sentence denotes An IgE-binding cDNA clone was isolated and heterologously expressed in Escherichia coli.
TextSentencer_T9 736-866 Sentence denotes The frequency of IgE-binding to the expressed protein and the IgE reactivity to allergen subunits were analyzed by immunoblotting.
T9 736-866 Sentence denotes The frequency of IgE-binding to the expressed protein and the IgE reactivity to allergen subunits were analyzed by immunoblotting.
TextSentencer_T10 867-875 Sentence denotes RESULTS:
T10 867-875 Sentence denotes RESULTS:
TextSentencer_T11 876-948 Sentence denotes An IgE-reactive cDNA clone (clone B) was isolated by plaque immunoassay.
T11 876-948 Sentence denotes An IgE-reactive cDNA clone (clone B) was isolated by plaque immunoassay.
TextSentencer_T12 949-1067 Sentence denotes The cDNA insert is 876-bp long and encodes a 228-amino acid polypeptide with a calculated molecular mass of 25 035 Da.
T12 949-1067 Sentence denotes The cDNA insert is 876-bp long and encodes a 228-amino acid polypeptide with a calculated molecular mass of 25 035 Da.
TextSentencer_T13 1068-1357 Sentence denotes Protein database search with the deduced clone B sequence revealed that 121 (53%) and 82 (36%) of the 228 amino acids were identical to those of the elongation factor 1-beta (EF-1beta) proteins from the yeast Saccharomyces cerevisiae and the parasite Echinococcus granulosus, respectively.
T13 1068-1357 Sentence denotes Protein database search with the deduced clone B sequence revealed that 121 (53%) and 82 (36%) of the 228 amino acids were identical to those of the elongation factor 1-beta (EF-1beta) proteins from the yeast Saccharomyces cerevisiae and the parasite Echinococcus granulosus, respectively.
TextSentencer_T14 1358-1440 Sentence denotes His-tagged recombinant clone B proteins were constructed and expressed in E. coli.
T14 1358-1440 Sentence denotes His-tagged recombinant clone B proteins were constructed and expressed in E. coli.
TextSentencer_T15 1441-1566 Sentence denotes Seven (8%) of the 92 serum samples from patients with bronchial asthma showed IgE-binding to the recombinant clone B protein.
T15 1441-1566 Sentence denotes Seven (8%) of the 92 serum samples from patients with bronchial asthma showed IgE-binding to the recombinant clone B protein.
TextSentencer_T16 1567-1809 Sentence denotes Among these seven positive sera, five demonstrated IgE-binding to the C-terminal fragment (aa 119-228) while the other two sera showed IgE reactivity to the N-terminal fragment (aa 1-118) of this newly identified EF-1betaPenicillium allergen.
T16 1567-1809 Sentence denotes Among these seven positive sera, five demonstrated IgE-binding to the C-terminal fragment (aa 119-228) while the other two sera showed IgE reactivity to the N-terminal fragment (aa 1-118) of this newly identified EF-1betaPenicillium allergen.
TextSentencer_T17 1810-1822 Sentence denotes CONCLUSIONS:
T17 1810-1822 Sentence denotes CONCLUSIONS:
TextSentencer_T18 1823-1917 Sentence denotes A novel P. citrinum allergen (Pen c 24) was identified and characterized in the present study.
T18 1823-1917 Sentence denotes A novel P. citrinum allergen (Pen c 24) was identified and characterized in the present study.
TextSentencer_T19 1918-2113 Sentence denotes Results obtained provide more information about allergens of prevalent airborne fungi and a basis to understand more about the IgE responses in human atopic disorders and in parasitic infections.
T19 1918-2113 Sentence denotes Results obtained provide more information about allergens of prevalent airborne fungi and a basis to understand more about the IgE responses in human atopic disorders and in parasitic infections.