| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-56 |
Sentence |
denotes |
Glycosylation-related gene expression in prion diseases: |
| T2 |
57-160 |
Sentence |
denotes |
PrPSc accumulation in scrapie infected GT1 cells depends on beta-1,4-linked GalNAc-4-SO4 hyposulfation. |
| T3 |
161-334 |
Sentence |
denotes |
Several lines of evidence indicate that some glycoconjugates are efficient effectors of the cellular prion protein (PrP(C)) conversion into its pathogenic (PrP(Sc)) isoform. |
| T4 |
335-672 |
Sentence |
denotes |
To assess how glycoconjugate glycan moieties participate in the biogenesis of PrP(Sc), an exhaustive comparative analysis of the expression of about 200 glycosylation-related genes was performed on prion-infected or not, hypothalamus-derived GT1 cells by hybridization of DNA microarrays, semiquantitative RT-PCR, and biochemical assays. |
| T5 |
673-831 |
Sentence |
denotes |
A significant up- (30-fold) and down- (17-fold) regulation of the expression of the ChGn1 and Chst8 genes, respectively, was observed in prion-infected cells. |
| T6 |
832-1005 |
Sentence |
denotes |
ChGn1 and Chst8 are involved in the initiation of the synthesis of chondroitin sulfate and in the 4-O-sulfation of non-reducing N-acetylgalactosamine residues, respectively. |
| T7 |
1006-1176 |
Sentence |
denotes |
A possible role for a hyposulfated chondroitin in PrP(Sc) accumulation was evidenced at the protein level and by determination of chondroitin and heparan sulfate amounts. |
| T8 |
1177-1407 |
Sentence |
denotes |
Treatment of Sc-GT1 cells with a heparan mimetic (HM2602) induced an important reduction of the amount of PrP(Sc), associated with a total reversion of the transcription pattern of the N-acetylgalactosamine-4-O-sulfotransferase 8. |
| T9 |
1408-1497 |
Sentence |
denotes |
It suggests a link between the genetic control of 4-O-sulfation and PrP(Sc) accumulation. |
