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PubMed:15632136 JSONTXT 24 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-97 Sentence denotes GPI7 is the second partner of PIG-F and involved in modification of glycosylphosphatidylinositol.
T2 98-204 Sentence denotes Many eukaryotic cell surface proteins are anchored to the membrane via glycosylphosphatidylinositol (GPI).
T3 205-313 Sentence denotes GPI is synthesized from phosphatidylinositol by stepwise reactions and attached en bloc to nascent proteins.
T4 314-393 Sentence denotes In mammalian cells, the major GPI species transferred to proteins is termed H7.
T5 394-604 Sentence denotes By attachment of an additional ethanolamine phosphate (EtNP) to the second mannose, H7 can be converted to H8, which acts as a minor type of protein-linked GPI and also exists as a free GPI on the cell surface.
T6 605-741 Sentence denotes Yeast GPI7 is involved in the transfer of EtNP to the second mannose, but the corresponding mammalian enzyme has not yet been clarified.
T7 742-876 Sentence denotes Here, we report that the human homolog of Gpi7p (hGPI7) forms a protein complex with PIG-F and is involved in the H7-to-H8 conversion.
T8 877-978 Sentence denotes We knocked down hGPI7 by RNA interference and found that H7 accumulated with little production of H8.
T9 979-1153 Sentence denotes Immunoprecipitation experiments revealed that hGPI7 was associated with and stabilized by PIG-F, which is known to bind to and stabilize PIG-O, a protein homologous to hGPI7.
T10 1154-1261 Sentence denotes PIG-O is a transferase that adds EtNP to the third mannose, rendering GPI capable of attaching to proteins.
T11 1262-1418 Sentence denotes We further found that the overexpression of hGPI7 decreased the level of PIG-O and, therefore, decreased the level of EtNP transferred to the third mannose.
T12 1419-1597 Sentence denotes Finally, we propose a mechanism for the regulation of GPI biosynthesis through competition between the two independent enzymes, PIG-O and hGPI7, for the common stabilizer, PIG-F.