| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T3 |
37-147 |
Sentence |
denotes |
We have shown previously that mice lacking the Nrf2 are more susceptible to hyperoxia than are wild-type mice. |
| T4 |
148-277 |
Sentence |
denotes |
Nrf2 activates antioxidant response element (ARE)-mediated gene expression involved in cellular protection against toxic insults. |
| T5 |
278-452 |
Sentence |
denotes |
The present study was designed to investigate the mechanisms that control the activation of Nrf2 by hyperoxia using a non-malignant murine alveolar epithelial cell line, C10. |
| T6 |
453-560 |
Sentence |
denotes |
No significant alteration in the levels of Nrf2 mRNA and protein was found following exposure to hyperoxia. |
| T7 |
561-680 |
Sentence |
denotes |
In contrast, hyperoxia caused the translocation of Nrf2 from the cytoplasm to the nucleus within 30-60 min of exposure. |
| T8 |
681-889 |
Sentence |
denotes |
Consistent with these observations, gel shift and reporter analyses demonstrated a correlation between the hyperoxia-enhanced ARE DNA-binding activity of Nrf2 and an up-regulation of ARE-driven transcription. |
| T9 |
890-1012 |
Sentence |
denotes |
Inhibition of NADPH oxidase with diphenyleneiodonium (DPI) blocked both Nrf2 translocation and ARE-mediated transcription. |
| T10 |
1013-1071 |
Sentence |
denotes |
Inhibition of the MEK/ERK pathway caused a similar effect. |
| T11 |
1072-1215 |
Sentence |
denotes |
Consistent with this finding, hyperoxia stimulated ERK-1 and ERK-2 phosphorylation, whereas DPI or N-acetyl-l-cysteine blocked such activation. |
| T12 |
1216-1382 |
Sentence |
denotes |
Hyperoxia stimulated the phosphorylation of endogenous Nrf2, but not in the presence of U0126, suggesting a critical role for ERK signaling in the activation of Nrf2. |
| T13 |
1383-1501 |
Sentence |
denotes |
Consistent with this notion, hyperoxia did not stimulate the phosphorylation of Nrf2 in fibroblasts lacking the ERK-1. |
