sentences Home  

PubMed:15292179 / 160-1761 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T3 41-151 Sentence denotes We have shown previously that mice lacking the Nrf2 are more susceptible to hyperoxia than are wild-type mice.
T4 152-281 Sentence denotes Nrf2 activates antioxidant response element (ARE)-mediated gene expression involved in cellular protection against toxic insults.
T5 282-456 Sentence denotes The present study was designed to investigate the mechanisms that control the activation of Nrf2 by hyperoxia using a non-malignant murine alveolar epithelial cell line, C10.
T6 457-564 Sentence denotes No significant alteration in the levels of Nrf2 mRNA and protein was found following exposure to hyperoxia.
T7 565-684 Sentence denotes In contrast, hyperoxia caused the translocation of Nrf2 from the cytoplasm to the nucleus within 30-60 min of exposure.
T8 685-893 Sentence denotes Consistent with these observations, gel shift and reporter analyses demonstrated a correlation between the hyperoxia-enhanced ARE DNA-binding activity of Nrf2 and an up-regulation of ARE-driven transcription.
T9 894-1016 Sentence denotes Inhibition of NADPH oxidase with diphenyleneiodonium (DPI) blocked both Nrf2 translocation and ARE-mediated transcription.
T10 1017-1075 Sentence denotes Inhibition of the MEK/ERK pathway caused a similar effect.
T11 1076-1219 Sentence denotes Consistent with this finding, hyperoxia stimulated ERK-1 and ERK-2 phosphorylation, whereas DPI or N-acetyl-l-cysteine blocked such activation.
T12 1220-1386 Sentence denotes Hyperoxia stimulated the phosphorylation of endogenous Nrf2, but not in the presence of U0126, suggesting a critical role for ERK signaling in the activation of Nrf2.
T13 1387-1505 Sentence denotes Consistent with this notion, hyperoxia did not stimulate the phosphorylation of Nrf2 in fibroblasts lacking the ERK-1.