> top > projects > sentences > docs > PubMed:15292179 > annotations
sentences Home  

PubMed:15292179 JSONTXT 21 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-124 Sentence denotes NADPH oxidase and ERK signaling regulates hyperoxia-induced Nrf2-ARE transcriptional response in pulmonary epithelial cells.
T2 125-200 Sentence denotes Oxidative stress plays a major role in hyperoxia-induced acute lung injury.
T3 201-311 Sentence denotes We have shown previously that mice lacking the Nrf2 are more susceptible to hyperoxia than are wild-type mice.
T4 312-441 Sentence denotes Nrf2 activates antioxidant response element (ARE)-mediated gene expression involved in cellular protection against toxic insults.
T5 442-616 Sentence denotes The present study was designed to investigate the mechanisms that control the activation of Nrf2 by hyperoxia using a non-malignant murine alveolar epithelial cell line, C10.
T6 617-724 Sentence denotes No significant alteration in the levels of Nrf2 mRNA and protein was found following exposure to hyperoxia.
T7 725-844 Sentence denotes In contrast, hyperoxia caused the translocation of Nrf2 from the cytoplasm to the nucleus within 30-60 min of exposure.
T8 845-1053 Sentence denotes Consistent with these observations, gel shift and reporter analyses demonstrated a correlation between the hyperoxia-enhanced ARE DNA-binding activity of Nrf2 and an up-regulation of ARE-driven transcription.
T9 1054-1176 Sentence denotes Inhibition of NADPH oxidase with diphenyleneiodonium (DPI) blocked both Nrf2 translocation and ARE-mediated transcription.
T10 1177-1235 Sentence denotes Inhibition of the MEK/ERK pathway caused a similar effect.
T11 1236-1379 Sentence denotes Consistent with this finding, hyperoxia stimulated ERK-1 and ERK-2 phosphorylation, whereas DPI or N-acetyl-l-cysteine blocked such activation.
T12 1380-1546 Sentence denotes Hyperoxia stimulated the phosphorylation of endogenous Nrf2, but not in the presence of U0126, suggesting a critical role for ERK signaling in the activation of Nrf2.
T13 1547-1665 Sentence denotes Consistent with this notion, hyperoxia did not stimulate the phosphorylation of Nrf2 in fibroblasts lacking the ERK-1.
T14 1666-1817 Sentence denotes Collectively, our findings suggest that hyperoxia-induced, ARE-driven, Nrf2-dependent transcription is controlled by NADPH oxidase and ERK-1 signaling.