> top > projects > sentences > docs > PubMed:15254034 > annotations
sentences Home  

PubMed:15254034 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-88 Sentence denotes Cytosolic NADP+-dependent isocitrate dehydrogenase plays a key role in lipid metabolism.
T2 89-219 Sentence denotes NADPH is an essential cofactor for many enzymatic reactions including glutathione metabolism and fat and cholesterol biosynthesis.
T3 220-440 Sentence denotes We have reported recently an important role for mitochondrial NADP(+)-dependent isocitrate dehydrogenase in cellular defense against oxidative damage by providing NADPH needed for the regeneration of reduced glutathione.
T4 441-539 Sentence denotes However, the role of cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDPc) is still unclear.
T5 540-642 Sentence denotes We report here for the first time that IDPc plays a critical role in fat and cholesterol biosynthesis.
T6 643-792 Sentence denotes During differentiation of 3T3-L1 adipocytes, both IDPc enzyme activity and its protein content were increased in parallel in a time-dependent manner.
T7 793-999 Sentence denotes Increased expression of IDPc by stable transfection of IDPc cDNA positively correlated with adipogenesis of 3T3-L1 cells, whereas decreased IDPc expression by an antisense IDPc vector retarded adipogenesis.
T8 1000-1104 Sentence denotes Furthermore, transgenic mice with overexpressed IDPc exhibited fatty liver, hyperlipidemia, and obesity.
T9 1105-1282 Sentence denotes In the epididymal fat pads of the transgenic mice, the expressions of adipocyte-specific genes including peroxisome proliferator-activated receptor gamma were markedly elevated.
T10 1283-1712 Sentence denotes The hepatic and epididymal fat pad contents of acetyl-CoA and malonyl-CoA in the transgenic mice were significantly lower, whereas the total triglyceride and cholesterol contents were markedly higher in the liver and serum of transgenic mice compared with those measured in wild type mice, suggesting that the consumption rate of those lipogenic precursors needed for fat biosynthesis must be increased by elevated IDPc activity.
T11 1713-1845 Sentence denotes Taken together, our findings strongly indicate that IDPc would be a major NADPH producer required for fat and cholesterol synthesis.