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PubMed:14722123 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-164 Sentence denotes N terminus is essential for tropomyosin functions: N-terminal modification disrupts stress fiber organization and abolishes anti-oncogenic effects of tropomyosin-1.
T2 165-381 Sentence denotes Down-regulation of several key actin-binding proteins, such as alpha-actinin, vinculin, gelsolin, and tropomyosins (TMs), is considered to contribute to the disorganized cytoskeleton present in many neoplastic cells.
T3 382-473 Sentence denotes TMs stabilize actin filaments against the gel severing actions of proteins such as cofilin.
T4 474-632 Sentence denotes Among multiple TMs expressed in non-muscle cells, tropomyosin-1 (TM1) isoform induces stress fibers and functions as a suppressor of malignant transformation.
T5 633-751 Sentence denotes However, the molecular mechanisms of TM1-mediated cytoskeletal effects and tumor suppression remain poorly understood.
T6 752-858 Sentence denotes We have hypothesized that the ability of TM1 to stabilize microfilaments is crucial for tumor suppression.
T7 859-1040 Sentence denotes In this study, by employing a variant TM1, which contains an N-terminal hemagglutinin epitope tag, we demonstrate that the N terminus is a key determinant of tropomyosin-1 function.
T8 1041-1181 Sentence denotes Unlike the wild type TM1, the modified protein fails to restore stress fibers and inhibit anchorage-independent growth in transformed cells.
T9 1182-1376 Sentence denotes Furthermore, the N-terminal modification of TM1 disorganizes the cytoskeleton and delays cytokinesis in normal cells, abolishes binding to F-actin, and disrupts the dimeric associations in vivo.
T10 1377-1578 Sentence denotes The functionally defective TM1 allows the association of cofilin to stress fibers and disorganizes the microfilaments, whereas wild type TM1 appears to restrict the binding of cofilin to stress fibers.
T11 1579-1701 Sentence denotes TM1-induced cytoskeletal reorganization appears to be mediated through preventing cofilin interaction with microfilaments.
T12 1702-1873 Sentence denotes Our studies provide in vivo functional evidence that the N terminus is a critical determinant of TM1 functions, which in turn determines the organization of stress fibers.