| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-82 |
Sentence |
denotes |
Control of human PIRH2 protein stability: involvement of TIP60 and the proteosome. |
| T2 |
83-482 |
Sentence |
denotes |
Murine PIRH2 (mPIRH2) was recently identified as a RING finger-containing ubiquitin-protein isopeptide ligase that interacts with both p53 and the human androgen receptor. mpirh2 is a p53-responsive gene that is up-regulated by UV, and mPIRH2 protein has the capacity to polyubiquitylate p53, perhaps leading to p53 destruction. mpirh2 therefore has properties similar to those of the oncogene mdm2. |
| T3 |
483-560 |
Sentence |
denotes |
Here, we have identified human PIRH2 (hPIRH2) as a TIP60-interacting protein. |
| T4 |
561-704 |
Sentence |
denotes |
To investigate its regulation, we characterized hPIRH2 in parallel with hPIRH2 variants possessing mutations of conserved RING finger residues. |
| T5 |
705-851 |
Sentence |
denotes |
We observed that wild-type hPIRH2 is an unstable protein with a short half-life and is a target for RING domain-dependent proteasomal degradation. |
| T6 |
852-913 |
Sentence |
denotes |
Accordingly, we found that hPIRH2 was ubiquitylated in cells. |
| T7 |
914-1082 |
Sentence |
denotes |
The TIP60-hPIRH2 association appeared to regulate hPIRH2 stability; coexpression of TIP60 enhanced hPIRH2 protein stability and altered hPIRH2 subcellular localization. |
| T8 |
1083-1197 |
Sentence |
denotes |
These results suggest that hPIRH2 activities can be controlled, at the post-translational level, in multiple ways. |
