| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-60 |
Sentence |
denotes |
Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. |
| T1 |
0-60 |
Sentence |
denotes |
Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. |
| T2 |
61-114 |
Sentence |
denotes |
The presence of sialyl Le(x) structures in O-glycans. |
| T2 |
61-114 |
Sentence |
denotes |
The presence of sialyl Le(x) structures in O-glycans. |
| T3 |
115-226 |
Sentence |
denotes |
Poly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. |
| T3 |
115-226 |
Sentence |
denotes |
Poly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. |
| T4 |
227-377 |
Sentence |
denotes |
Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. |
| T4 |
227-377 |
Sentence |
denotes |
Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. |
| T5 |
378-463 |
Sentence |
denotes |
Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. |
| T5 |
378-463 |
Sentence |
denotes |
Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. |
| T6 |
464-615 |
Sentence |
denotes |
Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. |
| T6 |
464-615 |
Sentence |
denotes |
Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. |
| T7 |
616-788 |
Sentence |
denotes |
The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. |
| T7 |
616-788 |
Sentence |
denotes |
The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. |
| T8 |
789-936 |
Sentence |
denotes |
Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. |
| T8 |
789-936 |
Sentence |
denotes |
Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. |
| T9 |
937-1039 |
Sentence |
denotes |
1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. |
| T9 |
937-1039 |
Sentence |
denotes |
1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. |
| T10 |
1040-1196 |
Sentence |
denotes |
2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2-->3Gal beta 1-->4 (Fuc alpha 1-->3)GlcNAc beta 1-->R. |
| T10 |
1040-1196 |
Sentence |
denotes |
2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2-->3Gal beta 1-->4 (Fuc alpha 1-->3)GlcNAc beta 1-->R. |
| T11 |
1197-1311 |
Sentence |
denotes |
The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. |
| T11 |
1197-1311 |
Sentence |
denotes |
The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. |
| T12 |
1312-1432 |
Sentence |
denotes |
3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. |
| T12 |
1312-1432 |
Sentence |
denotes |
3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. |
| T13 |
1433-1559 |
Sentence |
denotes |
4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. |
| T13 |
1433-1559 |
Sentence |
denotes |
4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. |
| T14 |
1560-1808 |
Sentence |
denotes |
5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2-->3Gal beta 1-->3(NeuNAc alpha 2-->6) GalNAc but not the hexasaccharide NeuNAc alpha 2-->3Gal beta 1-->3(NeuNAc alpha 2-->3Gal beta 1-->4GlcNAc beta 1-->6) GalNAc. |
| T14 |
1560-1808 |
Sentence |
denotes |
5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2-->3Gal beta 1-->3(NeuNAc alpha 2-->6) GalNAc but not the hexasaccharide NeuNAc alpha 2-->3Gal beta 1-->3(NeuNAc alpha 2-->3Gal beta 1-->4GlcNAc beta 1-->6) GalNAc. |
| T15 |
1809-1958 |
Sentence |
denotes |
These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation. |
| T15 |
1809-1958 |
Sentence |
denotes |
These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation. |