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PubMed:1328224 JSONTXT 24 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-68 Sentence denotes Biochemical properties of the 75-kDa tumor necrosis factor receptor.
T1 0-68 Sentence denotes Biochemical properties of the 75-kDa tumor necrosis factor receptor.
T2 69-151 Sentence denotes Characterization of ligand binding, internalization, and receptor phosphorylation.
T2 69-151 Sentence denotes Characterization of ligand binding, internalization, and receptor phosphorylation.
T3 152-350 Sentence denotes An expression plasmid encoding the human 75-kDa tumor necrosis factor (TNF) type 2 receptor (TNF-R2) was constructed and used to generate a stable human cell line (293/TNF-R2) overexpressing TNF-R2.
T3 152-350 Sentence denotes An expression plasmid encoding the human 75-kDa tumor necrosis factor (TNF) type 2 receptor (TNF-R2) was constructed and used to generate a stable human cell line (293/TNF-R2) overexpressing TNF-R2.
T4 351-587 Sentence denotes Ligand binding analysis revealed high affinity binding (Kd = 0.2 nM) with approximately 94,000 +/- 7,500 sites/cell for 125I-TNF-alpha and approximately 5-fold lower affinity for TNF-beta (Kd = 1.1 nM) with 264,000 +/- 2,000 sites/cell.
T4 351-587 Sentence denotes Ligand binding analysis revealed high affinity binding (Kd = 0.2 nM) with approximately 94,000 +/- 7,500 sites/cell for 125I-TNF-alpha and approximately 5-fold lower affinity for TNF-beta (Kd = 1.1 nM) with 264,000 +/- 2,000 sites/cell.
T5 588-848 Sentence denotes Cross-linking of 125I-TNF-alpha and 125I-TNF-beta to 293/TNF-R2 cells yielded predominant complexes with apparent molecular weights of 211,000 for TNF-alpha and 205,000 and 244,000 for TNF-beta, suggesting these complexes contain two or three TNF-R2 molecules.
T5 588-848 Sentence denotes Cross-linking of 125I-TNF-alpha and 125I-TNF-beta to 293/TNF-R2 cells yielded predominant complexes with apparent molecular weights of 211,000 for TNF-alpha and 205,000 and 244,000 for TNF-beta, suggesting these complexes contain two or three TNF-R2 molecules.
T6 849-962 Sentence denotes Immunoprecipitation of TNF-R2 from 32P-labeled 293/TNF-R2 cells demonstrated that the receptor is phosphorylated.
T6 849-962 Sentence denotes Immunoprecipitation of TNF-R2 from 32P-labeled 293/TNF-R2 cells demonstrated that the receptor is phosphorylated.
T7 963-1099 Sentence denotes The majority (97%) of 32Pi incorporation was found in serine residues with a very low level of incorporation (3%) in threonine residues.
T7 963-1099 Sentence denotes The majority (97%) of 32Pi incorporation was found in serine residues with a very low level of incorporation (3%) in threonine residues.
T8 1100-1210 Sentence denotes TNF-alpha treatment of 293/TNF-R2 cells did not significantly affect the degree or pattern of phosphorylation.
T8 1100-1210 Sentence denotes TNF-alpha treatment of 293/TNF-R2 cells did not significantly affect the degree or pattern of phosphorylation.
T9 1211-1318 Sentence denotes Cell surface-bound 125I-TNF-alpha was slowly internalized by the 293/TNF-R2 cell line with a t1/2 = 25 min.
T9 1211-1318 Sentence denotes Cell surface-bound 125I-TNF-alpha was slowly internalized by the 293/TNF-R2 cell line with a t1/2 = 25 min.
T10 1319-1453 Sentence denotes Shedding of the extracellular domain of TNF-R2 was induced by 4 beta-phorbol 12-myristate 13-acetate but not by TNF-alpha or TNF-beta.
T10 1319-1453 Sentence denotes Shedding of the extracellular domain of TNF-R2 was induced by 4 beta-phorbol 12-myristate 13-acetate but not by TNF-alpha or TNF-beta.