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PubMed:1321146 JSONTXT 22 Projects

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Id Subject Object Predicate Lexical cue
T1 0-134 Sentence denotes Purification and characterization of mitogen-activated protein kinase activator(s) from epidermal growth factor-stimulated A431 cells.
T1 0-134 Sentence denotes Purification and characterization of mitogen-activated protein kinase activator(s) from epidermal growth factor-stimulated A431 cells.
T2 135-325 Sentence denotes Two peaks of mitogen-activated protein (MAP) kinase activator activity are resolved upon ion exchange chromatography of cytosolic extracts from epidermal growth factor-stimulated A431 cells.
T2 135-325 Sentence denotes Two peaks of mitogen-activated protein (MAP) kinase activator activity are resolved upon ion exchange chromatography of cytosolic extracts from epidermal growth factor-stimulated A431 cells.
T3 326-520 Sentence denotes Two forms of the activator (1 and 2) have been purified from these peaks, using chromatography on Q-Sepharose, heparin-agarose, hydroxylapatite, ATP-agarose, Sephacryl S-300, Mono S, and Mono Q.
T3 326-520 Sentence denotes Two forms of the activator (1 and 2) have been purified from these peaks, using chromatography on Q-Sepharose, heparin-agarose, hydroxylapatite, ATP-agarose, Sephacryl S-300, Mono S, and Mono Q.
T4 521-704 Sentence denotes The two preparations each contained one major protein band with an apparent molecular mass of 46 or 45 kDa, respectively, on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T4 521-704 Sentence denotes The two preparations each contained one major protein band with an apparent molecular mass of 46 or 45 kDa, respectively, on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T5 705-796 Sentence denotes Evidence identifying the MAP kinase activators as the 46- and 45-kDa proteins is presented.
T5 705-796 Sentence denotes Evidence identifying the MAP kinase activators as the 46- and 45-kDa proteins is presented.
T6 797-1026 Sentence denotes Using inactive mutants of MAP kinase as potential substrates, it was found that each preparation of MAP kinase activator catalyzes phosphorylation of the regulatory residues, threonine 188 and tyrosine 190, of Xenopus MAP kinase.
T6 797-1026 Sentence denotes Using inactive mutants of MAP kinase as potential substrates, it was found that each preparation of MAP kinase activator catalyzes phosphorylation of the regulatory residues, threonine 188 and tyrosine 190, of Xenopus MAP kinase.
T7 1027-1112 Sentence denotes These results support the concept that the MAP kinase activators are protein kinases.
T7 1027-1112 Sentence denotes These results support the concept that the MAP kinase activators are protein kinases.
T8 1113-1410 Sentence denotes These MAP kinase kinases demonstrate an apparent high degree of specificity toward the native conformation of MAP kinase, although slow autophosphorylation on serine, threonine, and tyrosine residues and phosphorylation of myelin basic protein on serine and threonine residues is detected as well.
T8 1113-1410 Sentence denotes These MAP kinase kinases demonstrate an apparent high degree of specificity toward the native conformation of MAP kinase, although slow autophosphorylation on serine, threonine, and tyrosine residues and phosphorylation of myelin basic protein on serine and threonine residues is detected as well.