| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-54 |
Sentence |
denotes |
The three-dimensional structures of two beta-agarases. |
| T2 |
55-134 |
Sentence |
denotes |
Agars are important gelifying agents for biochemical use and the food industry. |
| T3 |
135-349 |
Sentence |
denotes |
To cleave the beta-1,4-linkages between beta-d-galactose and alpha-l-3,6-anhydro-galactose residues in the red algal galactans known as agars, marine bacteria produce polysaccharide hydrolases called beta-agarases. |
| T4 |
350-454 |
Sentence |
denotes |
Beta-agarases A and B from Zobellia galactanivorans Dsij have recently been biochemically characterized. |
| T5 |
455-525 |
Sentence |
denotes |
Here we report the first crystal structure of these two beta-agarases. |
| T6 |
526-700 |
Sentence |
denotes |
The two proteins were overproduced in Escherichia coli and crystallized, and the crystal structures were determined at 1.48 and 2.3 A for beta-agarases A and B, respectively. |
| T7 |
701-881 |
Sentence |
denotes |
The structure of beta-agarase A was solved by the multiple anomalous diffraction method, whereas beta-agarase B was solved with molecular replacement using beta-agarase A as model. |
| T8 |
882-1130 |
Sentence |
denotes |
Their structures adopt a jelly roll fold with a deep active site channel harboring the catalytic machinery, namely the nucleophilic residues Glu-147 and Glu-184 and the acid/base residues Glu-152 and Glu-189 for beta-agarases A and B, respectively. |
| T9 |
1131-1387 |
Sentence |
denotes |
The structures of the agarases were compared with those of two lichenases and of a kappa-carrageenase, which all belong to family 16 of the glycoside hydrolases in order to pinpoint the residues responsible for their widely differing substrate specificity. |
| T10 |
1388-1593 |
Sentence |
denotes |
The relationship between structure and enzymatic activity of the two beta-agarases from Z. galactanivorans Dsij was studied by analysis of the degradation products starting with different oligosaccharides. |
| T11 |
1594-1756 |
Sentence |
denotes |
The combination of the structural and biochemical results allowed the determination of the number of subsites present in the catalytic cleft of the beta-agarases. |
