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PubMed:12682047 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-107 Sentence denotes YMR313c/TGL3 encodes a novel triacylglycerol lipase located in lipid particles of Saccharomyces cerevisiae.
T2 108-202 Sentence denotes Previous work from our laboratory (Athenstaedt, K., Zweytick, D., Jandrositz, A., Kohlwein, S.
T3 203-229 Sentence denotes D., and Daum, G. (1999) J.
T4 230-240 Sentence denotes Bacteriol.
T5 241-445 Sentence denotes 181, 6441-6448) showed that the gene product of YMR313c (named Tgl3p) is a component of yeast lipid particles, and deletion of this gene led to an increase in the cellular level of triacylglycerols (TAG).
T6 446-537 Sentence denotes These observations suggested that TGL3 may encode a TAG lipase of Saccharomyces cerevisiae.
T7 538-764 Sentence denotes Here we demonstrate by cell fractionation and by microscopic inspection of a strain bearing a Tgl3p-GFP hybrid that this polypeptide is highly enriched in the lipid particle fraction but virtually absent from other organelles.
T8 765-1001 Sentence denotes The entire TAG lipase activity of lipid particles is attributed to Tgl3p, because the activity in this organelle is completely absent in a Deltatgl3 deletion mutant, whereas it is significantly enhanced in a strain overexpressing Tgl3p.
T9 1002-1149 Sentence denotes A His6-tagged Tgl3p hybrid purified close to homogeneity from a yeast strain overexpressing this fusion protein exhibited high TAG lipase activity.
T10 1150-1339 Sentence denotes Most importantly, experiments in vivo using the fatty acid synthesis inhibitor cerulenin demonstrated that deletion of TGL3 resulted in a decreased mobilization of TAG from lipid particles.
T11 1340-1482 Sentence denotes The amino acid sequence deduced from the open reading frame YMR313c contains the consensus sequence motif GXSXG typical for lipolytic enzymes.
T12 1483-1572 Sentence denotes Otherwise, Tgl3p has no significant sequence homology to other lipases identified so far.
T13 1573-1699 Sentence denotes In summary, our data identified Tgl3p as a novel yeast TAG lipase at the molecular level and by function in vivo and in vitro.