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PubMed:12646839 JSONTXT 13 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-127 Sentence denotes Gene discovery using a human vestibular schwannoma cDNA library constructed from a patient with neurofibromatosis type 2 (NF2).
T1 0-127 Sentence denotes Gene discovery using a human vestibular schwannoma cDNA library constructed from a patient with neurofibromatosis type 2 (NF2).
TextSentencer_T2 128-139 Sentence denotes BACKGROUND:
T2 128-139 Sentence denotes BACKGROUND:
TextSentencer_T3 140-327 Sentence denotes Despite a strong association of schwannomin/merlin gene mutations with vestibular schwannoma formation, the regulatory mechanisms and biologic pathways involved are still largely unknown.
T3 140-327 Sentence denotes Despite a strong association of schwannomin/merlin gene mutations with vestibular schwannoma formation, the regulatory mechanisms and biologic pathways involved are still largely unknown.
TextSentencer_T4 328-725 Sentence denotes The hypothesis of this study is that the genesis and growth characteristics of neurofibromatosis type 2 (NF2)-associated vestibular schwannomas are determined by genetic alterations that vary in gene transcript expression; this transcript expression includes oncogenic gene products that may be identified by construction and sequencing of a cDNA library from NF2-associated vestibular schwannoma.
T4 328-725 Sentence denotes The hypothesis of this study is that the genesis and growth characteristics of neurofibromatosis type 2 (NF2)-associated vestibular schwannomas are determined by genetic alterations that vary in gene transcript expression; this transcript expression includes oncogenic gene products that may be identified by construction and sequencing of a cDNA library from NF2-associated vestibular schwannoma.
TextSentencer_T5 726-734 Sentence denotes METHODS:
T5 726-734 Sentence denotes METHODS:
TextSentencer_T6 735-855 Sentence denotes Approximately 3 mL of fresh tumor was obtained during resection of a 4-cm vestibular schwannoma from a patient with NF2.
T6 735-855 Sentence denotes Approximately 3 mL of fresh tumor was obtained during resection of a 4-cm vestibular schwannoma from a patient with NF2.
TextSentencer_T7 856-1020 Sentence denotes Poly(A)(+) mRNA was isolated, synthesized into double-stranded cDNA, and unidirectionally inserted into Uni-Zap XR (Stratagene, La Jolla, CA) bacteriophage vectors.
T7 856-1020 Sentence denotes Poly(A)(+) mRNA was isolated, synthesized into double-stranded cDNA, and unidirectionally inserted into Uni-Zap XR (Stratagene, La Jolla, CA) bacteriophage vectors.
TextSentencer_T8 1021-1291 Sentence denotes Bacteriophage vectors containing cDNA inserts were processed into phagemids according to Uni-Zap XR protocol, and inserted vectors were sequenced and analyzed using BLAST software (National Institutes of Health, Bethesda, MD) with GenBank, EMBL, DDBJ, and PBD databases.
T8 1021-1291 Sentence denotes Bacteriophage vectors containing cDNA inserts were processed into phagemids according to Uni-Zap XR protocol, and inserted vectors were sequenced and analyzed using BLAST software (National Institutes of Health, Bethesda, MD) with GenBank, EMBL, DDBJ, and PBD databases.
TextSentencer_T9 1292-1300 Sentence denotes RESULTS:
T9 1292-1300 Sentence denotes RESULTS:
TextSentencer_T10 1301-1356 Sentence denotes The cDNA library contained 2.4 million primary plaques.
T10 1301-1356 Sentence denotes The cDNA library contained 2.4 million primary plaques.
TextSentencer_T11 1357-1434 Sentence denotes Inserts averaged 1.8 kilobases (kb) in length, with a range of 0.8 to 3.0 kb.
T11 1357-1434 Sentence denotes Inserts averaged 1.8 kilobases (kb) in length, with a range of 0.8 to 3.0 kb.
TextSentencer_T12 1435-1668 Sentence denotes BLAST multidatabase comparison of the sequence data obtained from 50 randomly selected clones yielded identification of 13 sequences representing known human genes and 17 sequences representing cloned sequences with unknown function.
T12 1435-1668 Sentence denotes BLAST multidatabase comparison of the sequence data obtained from 50 randomly selected clones yielded identification of 13 sequences representing known human genes and 17 sequences representing cloned sequences with unknown function.
TextSentencer_T13 1669-1815 Sentence denotes Three clones represented sequences not previously described in vestibular schwannomas but strongly implicated in oncogenesis within other tissues.
T13 1669-1815 Sentence denotes Three clones represented sequences not previously described in vestibular schwannomas but strongly implicated in oncogenesis within other tissues.
TextSentencer_T14 1816-1828 Sentence denotes CONCLUSIONS:
T14 1816-1828 Sentence denotes CONCLUSIONS:
TextSentencer_T15 1829-1940 Sentence denotes These data have implications for understanding the molecular mechanisms of vestibular schwannoma tumor biology.
T15 1829-1940 Sentence denotes These data have implications for understanding the molecular mechanisms of vestibular schwannoma tumor biology.
TextSentencer_T16 1941-2031 Sentence denotes Identified genes may provide future diagnostic/prognostic markers and therapeutic targets.
T16 1941-2031 Sentence denotes Identified genes may provide future diagnostic/prognostic markers and therapeutic targets.