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PubMed:12626411 JSONTXT 73 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-123 Sentence denotes The two rat alpha 2,6-sialyltransferase (ST6Gal I) isoforms: evaluation of catalytic activity and intra-Golgi localization.
T1 0-123 Sentence denotes The two rat alpha 2,6-sialyltransferase (ST6Gal I) isoforms: evaluation of catalytic activity and intra-Golgi localization.
TextSentencer_T2 124-256 Sentence denotes alpha2,6-Sialyltransferase (ST6Gal I) functions in the Golgi to terminally sialylate the N-linked oligosaccharides of glycoproteins.
T2 124-256 Sentence denotes alpha2,6-Sialyltransferase (ST6Gal I) functions in the Golgi to terminally sialylate the N-linked oligosaccharides of glycoproteins.
TextSentencer_T3 257-394 Sentence denotes Interestingly, rat ST6Gal I is expressed as two isoforms, STtyr and STcys, that differ by a single amino acid in their catalytic domains.
T3 257-394 Sentence denotes Interestingly, rat ST6Gal I is expressed as two isoforms, STtyr and STcys, that differ by a single amino acid in their catalytic domains.
TextSentencer_T4 395-588 Sentence denotes In this article, our goal was to evaluate more carefully possible differences in the catalytic activity and intra-Golgi localization of the two isoforms that had been suggested by earlier work.
T4 395-588 Sentence denotes In this article, our goal was to evaluate more carefully possible differences in the catalytic activity and intra-Golgi localization of the two isoforms that had been suggested by earlier work.
TextSentencer_T5 589-782 Sentence denotes Using soluble recombinant STtyr and STcys enzymes and three asialoglycoprotein substrates for in vitro analysis, we found that the STcys isoform was somewhat more active than the STtyr isoform.
T5 589-782 Sentence denotes Using soluble recombinant STtyr and STcys enzymes and three asialoglycoprotein substrates for in vitro analysis, we found that the STcys isoform was somewhat more active than the STtyr isoform.
TextSentencer_T6 783-970 Sentence denotes However, we found no differences in isoform substrate choice when these proteins were expressed in Chinese hamster ovary cells, and sialylated substrates were detected by lectin blotting.
T6 783-970 Sentence denotes However, we found no differences in isoform substrate choice when these proteins were expressed in Chinese hamster ovary cells, and sialylated substrates were detected by lectin blotting.
TextSentencer_T7 971-1200 Sentence denotes Immuno-fluorescence and immunoelectron microscopy revealed differences in the relative levels of the isoforms found in the endoplasmic reticulum (ER) and Golgi of transiently expressing cells but similar intra-Golgi localization.
T7 971-1200 Sentence denotes Immuno-fluorescence and immunoelectron microscopy revealed differences in the relative levels of the isoforms found in the endoplasmic reticulum (ER) and Golgi of transiently expressing cells but similar intra-Golgi localization.
TextSentencer_T8 1201-1295 Sentence denotes STtyr was restricted to the Golgi in most cells, and STcys was found in both the ER and Golgi.
T8 1201-1295 Sentence denotes STtyr was restricted to the Golgi in most cells, and STcys was found in both the ER and Golgi.
TextSentencer_T9 1296-1384 Sentence denotes The ER localization of STcys was especially pronounced with a C-terminal V5 epitope tag.
T9 1296-1384 Sentence denotes The ER localization of STcys was especially pronounced with a C-terminal V5 epitope tag.
TextSentencer_T10 1385-1558 Sentence denotes Ultrastructural and deconvolution studies of immunostained HeLa cells expressing STtyr or STcys showed that within the Golgi both isoforms are found in medial-trans regions.
T10 1385-1558 Sentence denotes Ultrastructural and deconvolution studies of immunostained HeLa cells expressing STtyr or STcys showed that within the Golgi both isoforms are found in medial-trans regions.
TextSentencer_T11 1559-1784 Sentence denotes The similar catalytic activities and intra-Golgi localization of the two ST6Gal I isoforms suggest that the particular isoform expressed in specific cells and tissues is not likely to have significant functional consequences.
T11 1559-1784 Sentence denotes The similar catalytic activities and intra-Golgi localization of the two ST6Gal I isoforms suggest that the particular isoform expressed in specific cells and tissues is not likely to have significant functional consequences.