Id |
Subject |
Object |
Predicate |
Lexical cue |
TextSentencer_T1 |
0-123 |
Sentence |
denotes |
The two rat alpha 2,6-sialyltransferase (ST6Gal I) isoforms: evaluation of catalytic activity and intra-Golgi localization. |
T1 |
0-123 |
Sentence |
denotes |
The two rat alpha 2,6-sialyltransferase (ST6Gal I) isoforms: evaluation of catalytic activity and intra-Golgi localization. |
TextSentencer_T2 |
124-256 |
Sentence |
denotes |
alpha2,6-Sialyltransferase (ST6Gal I) functions in the Golgi to terminally sialylate the N-linked oligosaccharides of glycoproteins. |
T2 |
124-256 |
Sentence |
denotes |
alpha2,6-Sialyltransferase (ST6Gal I) functions in the Golgi to terminally sialylate the N-linked oligosaccharides of glycoproteins. |
TextSentencer_T3 |
257-394 |
Sentence |
denotes |
Interestingly, rat ST6Gal I is expressed as two isoforms, STtyr and STcys, that differ by a single amino acid in their catalytic domains. |
T3 |
257-394 |
Sentence |
denotes |
Interestingly, rat ST6Gal I is expressed as two isoforms, STtyr and STcys, that differ by a single amino acid in their catalytic domains. |
TextSentencer_T4 |
395-588 |
Sentence |
denotes |
In this article, our goal was to evaluate more carefully possible differences in the catalytic activity and intra-Golgi localization of the two isoforms that had been suggested by earlier work. |
T4 |
395-588 |
Sentence |
denotes |
In this article, our goal was to evaluate more carefully possible differences in the catalytic activity and intra-Golgi localization of the two isoforms that had been suggested by earlier work. |
TextSentencer_T5 |
589-782 |
Sentence |
denotes |
Using soluble recombinant STtyr and STcys enzymes and three asialoglycoprotein substrates for in vitro analysis, we found that the STcys isoform was somewhat more active than the STtyr isoform. |
T5 |
589-782 |
Sentence |
denotes |
Using soluble recombinant STtyr and STcys enzymes and three asialoglycoprotein substrates for in vitro analysis, we found that the STcys isoform was somewhat more active than the STtyr isoform. |
TextSentencer_T6 |
783-970 |
Sentence |
denotes |
However, we found no differences in isoform substrate choice when these proteins were expressed in Chinese hamster ovary cells, and sialylated substrates were detected by lectin blotting. |
T6 |
783-970 |
Sentence |
denotes |
However, we found no differences in isoform substrate choice when these proteins were expressed in Chinese hamster ovary cells, and sialylated substrates were detected by lectin blotting. |
TextSentencer_T7 |
971-1200 |
Sentence |
denotes |
Immuno-fluorescence and immunoelectron microscopy revealed differences in the relative levels of the isoforms found in the endoplasmic reticulum (ER) and Golgi of transiently expressing cells but similar intra-Golgi localization. |
T7 |
971-1200 |
Sentence |
denotes |
Immuno-fluorescence and immunoelectron microscopy revealed differences in the relative levels of the isoforms found in the endoplasmic reticulum (ER) and Golgi of transiently expressing cells but similar intra-Golgi localization. |
TextSentencer_T8 |
1201-1295 |
Sentence |
denotes |
STtyr was restricted to the Golgi in most cells, and STcys was found in both the ER and Golgi. |
T8 |
1201-1295 |
Sentence |
denotes |
STtyr was restricted to the Golgi in most cells, and STcys was found in both the ER and Golgi. |
TextSentencer_T9 |
1296-1384 |
Sentence |
denotes |
The ER localization of STcys was especially pronounced with a C-terminal V5 epitope tag. |
T9 |
1296-1384 |
Sentence |
denotes |
The ER localization of STcys was especially pronounced with a C-terminal V5 epitope tag. |
TextSentencer_T10 |
1385-1558 |
Sentence |
denotes |
Ultrastructural and deconvolution studies of immunostained HeLa cells expressing STtyr or STcys showed that within the Golgi both isoforms are found in medial-trans regions. |
T10 |
1385-1558 |
Sentence |
denotes |
Ultrastructural and deconvolution studies of immunostained HeLa cells expressing STtyr or STcys showed that within the Golgi both isoforms are found in medial-trans regions. |
TextSentencer_T11 |
1559-1784 |
Sentence |
denotes |
The similar catalytic activities and intra-Golgi localization of the two ST6Gal I isoforms suggest that the particular isoform expressed in specific cells and tissues is not likely to have significant functional consequences. |
T11 |
1559-1784 |
Sentence |
denotes |
The similar catalytic activities and intra-Golgi localization of the two ST6Gal I isoforms suggest that the particular isoform expressed in specific cells and tissues is not likely to have significant functional consequences. |