| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-82 |
Sentence |
denotes |
Beta 1,4-galactosyltransferase (beta 4GalT)-IV is specific for GlcNAc 6-O-sulfate. |
| T2 |
83-185 |
Sentence |
denotes |
Beta 4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X. |
| T3 |
186-356 |
Sentence |
denotes |
The Galbeta1-->4(SO(3)(-)-->6)GlcNAc moiety is present in various N-linked and O-linked glycans including keratan sulfate and 6-sulfosialyl-Lewis X, an L-selectin ligand. |
| T4 |
357-621 |
Sentence |
denotes |
We previously found beta1,4-galactosyltransferase (beta4GalT) activity in human colonic mucosa, which prefers GlcNAc 6-O-sulfate (6SGN) as an acceptor to non-substituted GlcNAc (Seko, A., Hara-Kuge, S., Nagata, K., Yonezawa, S., and Yamashita, K. (1998) FEBS Lett. |
| T5 |
622-636 |
Sentence |
denotes |
440, 307-310). |
| T6 |
637-726 |
Sentence |
denotes |
To identify the gene for this enzyme, we purified the enzyme from porcine colonic mucosa. |
| T7 |
727-821 |
Sentence |
denotes |
The purified enzyme had the characteristic requirement of basic lipids for catalytic activity. |
| T8 |
822-962 |
Sentence |
denotes |
Analysis of the partial amino acid sequence of the enzyme revealed that the purified beta4GalT has a similar sequence to human beta4GalT-IV. |
| T9 |
963-1165 |
Sentence |
denotes |
To confirm this result, we prepared cDNA for each of the seven beta4GalTs cloned to date and examined substrate specificities using the membrane fractions derived from beta4GalT-transfected COS-7 cells. |
| T10 |
1166-1462 |
Sentence |
denotes |
When using several N-linked and O-linked glycans with or without 6SGN residues as acceptor substrates, only beta4GalT-IV efficiently recognized 6SGN, keratan sulfate-related oligosaccharides, and Galbeta1-->3(SO(3)(-)-->6GlcNAcbeta1-->6) GalNAcalpha1-O-pNP, a precursor for 6-sulfosialyl-Lewis X. |
| T11 |
1463-1650 |
Sentence |
denotes |
These results suggested that beta4GalT-IV is a 6SGN-specific beta4GalT and may be involved in the biosynthesis of various glycoproteins carrying a 6-O-sulfated N-acetyllactosamine moiety. |
