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PubMed:12207025 JSONTXT 49 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-119 Sentence denotes Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate.
T2 120-155 Sentence denotes Insights into the enzyme mechanism.
T3 156-328 Sentence denotes The crystal structure of porcine heart mitochondrial NADP+-dependent isocitrate dehydrogenase (IDH) complexed with Mn2+ and isocitrate was solved to a resolution of 1.85 A.
T4 329-488 Sentence denotes The enzyme was expressed in Escherichia coli, purified as a fusion protein with maltose binding protein, and cleaved with thrombin to yield homogeneous enzyme.
T5 489-654 Sentence denotes The structure was determined by multiwavelength anomalous diffraction phasing using selenium substitution in the form of selenomethionine as the anomalous scatterer.
T6 655-861 Sentence denotes The porcine NADP+-IDH enzyme is structurally compared with the previously solved structures of IDH from E. coli and Bacillus subtilis that share 16 and 17% identity, respectively, with the mammalian enzyme.
T7 862-983 Sentence denotes The porcine enzyme has a protein fold similar to the bacterial IDH structures with each monomer folding into two domains.
T8 984-1120 Sentence denotes However, considerable differences exist between the bacterial and mammalian forms of IDH in regions connecting core secondary structure.
T9 1121-1296 Sentence denotes Based on the alignment of sequence and structure among the porcine, E. coli, and B. subtilis IDH, a putative phosphorylation site has been identified for the mammalian enzyme.
T10 1297-1421 Sentence denotes The active site, including the bound Mn2+-isocitrate complex, is highly ordered and, therefore, mechanistically informative.
T11 1422-1557 Sentence denotes The consensus IDH mechanism predicts that the Mn2+-bound hydroxyl of isocitrate is deprotonated prior to its NADP+-dependent oxidation.
T12 1558-1738 Sentence denotes The present crystal structure has an active site water that is well positioned to accept the proton and ultimately transfer the proton to solvent through an additional bound water.