| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-220 |
Sentence |
denotes |
Enzymatic synthesis of chondroitin with a novel chondroitin sulfate N-acetylgalactosaminyltransferase that transfers N-acetylgalactosamine to glucuronic acid in initiation and elongation of chondroitin sulfate synthesis. |
| T2 |
221-467 |
Sentence |
denotes |
We found a novel glycosyltransferase gene having a hypothetical beta 1,4-galactosyltransferase motif (GenBank accession number ) by a BLAST search and cloned its full-length open reading frame using the 5'-rapid amplification of cDNA ends method. |
| T3 |
468-537 |
Sentence |
denotes |
The truncated form was expressed in insect cells as a soluble enzyme. |
| T4 |
538-632 |
Sentence |
denotes |
It transferred N-acetylgalactosamine, not galactose, to para-nitrophenyl-beta-glucuronic acid. |
| T5 |
633-808 |
Sentence |
denotes |
The N-acetylgalactosamine-glucuronic acid linkage has been identified only in chondroitin sulfate; therefore, we examined its chondroitin elongation and initiation activities. |
| T6 |
809-1125 |
Sentence |
denotes |
N-Acetylgalactosaminyltransferase activity was observed toward chondroitin poly- and oligosaccharides, chondroitin sulfate oligosaccharides, and linkage tetrasaccharide (GlcA-Gal-Gal-Xyl-O-methoxyphenyl), and the chondroitin polysaccharide and linkage tetrasaccharide were better acceptor substrates than the others. |
| T7 |
1126-1342 |
Sentence |
denotes |
Northern blot analysis and quantitative real-time PCR analysis revealed that its 4-kb transcripts were highly expressed in thyroid and placenta, although they were ubiquitously expressed in various tissues and cells. |
| T8 |
1343-1500 |
Sentence |
denotes |
These results suggest that this enzyme has N-acetylgalactosaminyltransferase activity in both the elongation and initiation of chondroitin sulfate synthesis. |
| T9 |
1501-1587 |
Sentence |
denotes |
Furthermore, we performed enzymatic synthesis of chondroitin pentasaccharide in vitro. |
| T10 |
1588-1844 |
Sentence |
denotes |
In one tube reaction with four enzymes, beta 1,4-galactosyltransferase-VII, beta 1,3-galactosyltransferase-VI, glucuronyltransferase-I, and this enzyme, and a synthetic xylose-peptide acceptor, the structure GalNAc-GlcA-Gal-Gal-Xyl-peptide was constructed. |
| T11 |
1845-1962 |
Sentence |
denotes |
This is the first report of a chondroitin pentasaccharide constructed with recombinant glycosyltransferases in vitro. |
