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PubMed:12145278 JSONTXT 39 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-148 Sentence denotes Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine.
T2 149-294 Sentence denotes We found a novel human gene (GenBank accession number, Kazusa DNA Research Institute KIAA1402) that possesses homology with chondroitin synthase.
T3 295-405 Sentence denotes The full-length open reading frame consists of 772 amino acids and encodes a typical type II membrane protein.
T4 406-632 Sentence denotes This enzyme had a domain containing beta 3-glycosyltransferase motifs, which might be a beta3-glucuronyltransferase domain, but no domain with beta 4-glycosyltransferase motifs, although both are found in chondroitin synthase.
T5 633-712 Sentence denotes The putative catalytic domain was expressed in COS-7 cells as a soluble enzyme.
T6 713-872 Sentence denotes Its glucuronyltransferase activity was observed when chondroitin and chondroitin sulfate polysaccharides and oligosaccharides were used as acceptor substrates.
T7 873-1072 Sentence denotes However, it was not detected when dermatan sulfate, hyaluronan, heparan sulfate, heparin, N-acetylheparosan, lactosamine tetrasaccharide, and linkage tri- and tetrasaccharide acceptors were employed.
T8 1073-1234 Sentence denotes The reaction product, which was speculated to exhibit a GlcA beta 1-3GalNAc linkage structure at its non-reducing terminus, showed the following characteristics.
T9 1235-1279 Sentence denotes 1) It was catabolized by beta-glucuronidase.
T10 1280-1377 Sentence denotes 2) It was an acceptor for Escherichia coli K4 chondroitin polymerase (K4 chondroitin polymerase).
T11 1378-1457 Sentence denotes 3) The product of K4 chondroitin polymerase was cleaved by chondroitinase ACII.
T12 1458-1557 Sentence denotes On the other hand, no N-acetylgalactosaminyltransferase activity was detected toward any acceptors.
T13 1558-1770 Sentence denotes Quantitative real time PCR analysis revealed that its transcripts were highly expressed in the placenta, small intestine, and pancreas, although they were ubiquitously expressed in various tissues and cell lines.
T14 1771-1868 Sentence denotes This enzyme could play a role in the synthesis of chondroitin sulfate as a glucuronyltransferase.