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PubMed:12104089 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-80 Sentence denotes A restriction endonuclease assay for expression of human alpha-amylase isozymes.
T1 0-80 Sentence denotes A restriction endonuclease assay for expression of human alpha-amylase isozymes.
TextSentencer_T2 81-92 Sentence denotes BACKGROUND:
T2 81-92 Sentence denotes BACKGROUND:
TextSentencer_T3 93-254 Sentence denotes The alpha-amylase isozymes can be detected separately by electrophoresis; however, sometimes the identification is difficult because of their microheterogeneity.
T3 93-254 Sentence denotes The alpha-amylase isozymes can be detected separately by electrophoresis; however, sometimes the identification is difficult because of their microheterogeneity.
TextSentencer_T4 255-373 Sentence denotes In the present study, we tried to establish a convenient method for the detection of alpha-amylase isozyme expression.
T4 255-373 Sentence denotes In the present study, we tried to establish a convenient method for the detection of alpha-amylase isozyme expression.
TextSentencer_T5 374-382 Sentence denotes METHODS:
T5 374-382 Sentence denotes METHODS:
TextSentencer_T6 383-583 Sentence denotes The procedure is based on three different restriction sites presented in those genes; a PstI site in both AMY 2A and 2B genes, a HaeII site in both AMY 1 and 2A genes, and a BamHI site in AMY 2B gene.
T6 383-583 Sentence denotes The procedure is based on three different restriction sites presented in those genes; a PstI site in both AMY 2A and 2B genes, a HaeII site in both AMY 1 and 2A genes, and a BamHI site in AMY 2B gene.
TextSentencer_T7 584-756 Sentence denotes After amplification from total tissue RNAs by RT-PCR with primers that were able to cover each exon, the products were cleaved with corresponding restriction endonucleases.
T7 584-756 Sentence denotes After amplification from total tissue RNAs by RT-PCR with primers that were able to cover each exon, the products were cleaved with corresponding restriction endonucleases.
TextSentencer_T8 757-765 Sentence denotes RESULTS:
T8 757-765 Sentence denotes RESULTS:
TextSentencer_T9 766-891 Sentence denotes This method was applied to human samples from the parotid gland, liver (non-hepatoma), hepatoma and white blood cells (WBCs).
T9 766-891 Sentence denotes This method was applied to human samples from the parotid gland, liver (non-hepatoma), hepatoma and white blood cells (WBCs).
TextSentencer_T10 892-1018 Sentence denotes The results indicated that the parotid gland and hepatoma (also liver) clearly expressed AMY 1 and AMY 2B genes, respectively.
T10 892-1018 Sentence denotes The results indicated that the parotid gland and hepatoma (also liver) clearly expressed AMY 1 and AMY 2B genes, respectively.
TextSentencer_T11 1019-1216 Sentence denotes However, AMY 2B gene was also expressed apparently in WBCs, which produced salivary-type isozyme of the alpha-amylase, although the amylase protein was not able to identify for the hepatic isozyme.
T11 1019-1216 Sentence denotes However, AMY 2B gene was also expressed apparently in WBCs, which produced salivary-type isozyme of the alpha-amylase, although the amylase protein was not able to identify for the hepatic isozyme.
TextSentencer_T12 1217-1229 Sentence denotes CONCLUSIONS:
T12 1217-1229 Sentence denotes CONCLUSIONS:
TextSentencer_T13 1230-1355 Sentence denotes The method presented here might be convenient and useful for the determination of alpha-amylase isozyme expression in humans.
T13 1230-1355 Sentence denotes The method presented here might be convenient and useful for the determination of alpha-amylase isozyme expression in humans.