| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-100 |
Sentence |
denotes |
Identification of protein arginine methyltransferase 2 as a coactivator for estrogen receptor alpha. |
| T2 |
101-347 |
Sentence |
denotes |
In an attempt to isolate cofactors capable of influencing estrogen receptor alpha (ERalpha) transcriptional activity, we used yeast two-hybrid screening and identified protein arginine methyltransferase 2 (PRMT2) as a new ERalpha-binding protein. |
| T3 |
348-496 |
Sentence |
denotes |
PRMT2 interacted directly with three ERalpha regions including AF-1, DNA binding domain, and hormone binding domain in a ligand-independent fashion. |
| T4 |
497-598 |
Sentence |
denotes |
The ERalpha-interacting region on PRMT2 has been mapped to a region encompassing amino acids 133-275. |
| T5 |
599-704 |
Sentence |
denotes |
PRMT2 also binds to ERbeta, PR, TRbeta, RARalpha, PPARgamma, and RXRalpha in a ligand-independent manner. |
| T6 |
705-873 |
Sentence |
denotes |
PRMT2 enhanced both ERalpha AF-1 and AF-2 transcriptional activity, and the potential methyltransferase activity of PRMT2 appeared pivotal for its coactivator function. |
| T7 |
874-955 |
Sentence |
denotes |
In addition, PRMT2 enhanced PR, PPARgamma, and RARalpha-mediated transactivation. |
| T8 |
956-1273 |
Sentence |
denotes |
Although PRMT2 was found to interact with two other coactivators, the steroid receptor coactivator-1 (SRC-1) and the peroxisome proliferator-activated receptor-interacting protein (PRIP), no synergistic enhancement of ERalpha transcriptional activity was observed when PRMT2 was coexpressed with either PRIP or SRC-1. |
| T9 |
1274-1390 |
Sentence |
denotes |
In this respect PRMT2 differs from coactivators PRMT1 and CARM1 (coactivator-associated arginine methyltransferase). |
| T10 |
1391-1455 |
Sentence |
denotes |
These results suggest that PRMT2 is a novel ERalpha coactivator. |
