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PubMed:11996833 JSONTXT 21 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-102 Sentence denotes Studies on the glycosylation of wild-type and mutant forms of Aspergillus niger pectin methylesterase.
T1 0-102 Sentence denotes Studies on the glycosylation of wild-type and mutant forms of Aspergillus niger pectin methylesterase.
TextSentencer_T2 103-278 Sentence denotes Pectin methylesterase (PME) is one of a number of enzymes released by the fungus Aspergillus niger that are involved in the degradation of specific plant cell-wall structures.
T2 103-278 Sentence denotes Pectin methylesterase (PME) is one of a number of enzymes released by the fungus Aspergillus niger that are involved in the degradation of specific plant cell-wall structures.
TextSentencer_T3 279-355 Sentence denotes PME is a glycoprotein with three potential sites for N-linked glycosylation.
T3 279-355 Sentence denotes PME is a glycoprotein with three potential sites for N-linked glycosylation.
TextSentencer_T4 356-445 Sentence denotes The glycosylation may affect the hydrolytic activity or the substrate specificity of PME.
T4 356-445 Sentence denotes The glycosylation may affect the hydrolytic activity or the substrate specificity of PME.
TextSentencer_T5 446-573 Sentence denotes In this work, we investigate first the structures and the attachment sites of the glycans present on recombinant wild-type PME.
T5 446-573 Sentence denotes In this work, we investigate first the structures and the attachment sites of the glycans present on recombinant wild-type PME.
TextSentencer_T6 574-722 Sentence denotes Further, a series of PME mutants was created in which the three potential N-linked glycosylation sites were eliminated in all possible combinations.
T6 574-722 Sentence denotes Further, a series of PME mutants was created in which the three potential N-linked glycosylation sites were eliminated in all possible combinations.
TextSentencer_T7 723-795 Sentence denotes The glycosylation of the mutants and their activities were then studied.
T7 723-795 Sentence denotes The glycosylation of the mutants and their activities were then studied.
TextSentencer_T8 796-967 Sentence denotes Mass spectrometric techniques tailored for carbohydrate analysis were applied to both characterize the glycan structures and to determine the specific sites of attachment.
T8 796-967 Sentence denotes Mass spectrometric techniques tailored for carbohydrate analysis were applied to both characterize the glycan structures and to determine the specific sites of attachment.
TextSentencer_T9 968-1082 Sentence denotes High mannose structures with variable numbers of mannose were found on the wild-type, as well as the mutant forms.
T9 968-1082 Sentence denotes High mannose structures with variable numbers of mannose were found on the wild-type, as well as the mutant forms.
TextSentencer_T10 1083-1177 Sentence denotes Studies using the mutants suggest that glycosylation does not strongly influence the activity.
T10 1083-1177 Sentence denotes Studies using the mutants suggest that glycosylation does not strongly influence the activity.
TextSentencer_T11 1178-1296 Sentence denotes Whether it may affect the substrate specify of the enzyme is unknown, and that aspect will be explored in future work.
T11 1178-1296 Sentence denotes Whether it may affect the substrate specify of the enzyme is unknown, and that aspect will be explored in future work.