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PubMed:11942410 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-87 Sentence denotes The inhibition of lung cancer cell growth by intracellular immunization with LC-1 ScFv.
T1 0-87 Sentence denotes The inhibition of lung cancer cell growth by intracellular immunization with LC-1 ScFv.
TextSentencer_T2 88-204 Sentence denotes A monoclonal antibody, LC-1, recognizing lung cancer associated common antigens was obtained in authors' laboratory.
T2 88-204 Sentence denotes A monoclonal antibody, LC-1, recognizing lung cancer associated common antigens was obtained in authors' laboratory.
TextSentencer_T3 205-321 Sentence denotes Its single chain Fv fragment (ScFv) named LC-1 ScFv was constructed based on recombinant phage displayed techniques.
T3 205-321 Sentence denotes Its single chain Fv fragment (ScFv) named LC-1 ScFv was constructed based on recombinant phage displayed techniques.
TextSentencer_T4 322-471 Sentence denotes For expression on cell membrane, LC-1 ScFv was cloned into pDisplay vector, which directed the cloned gene to express as cell membrane bound protein.
T4 322-471 Sentence denotes For expression on cell membrane, LC-1 ScFv was cloned into pDisplay vector, which directed the cloned gene to express as cell membrane bound protein.
TextSentencer_T5 472-598 Sentence denotes The resulting plasmid was sequenced and then introduced by the lipofectin method into a lung adenocarcinoma cell line SPC-A-1.
T5 472-598 Sentence denotes The resulting plasmid was sequenced and then introduced by the lipofectin method into a lung adenocarcinoma cell line SPC-A-1.
TextSentencer_T6 599-652 Sentence denotes G418 resistant cells were obtained by G418 selection.
T6 599-652 Sentence denotes G418 resistant cells were obtained by G418 selection.
TextSentencer_T7 653-814 Sentence denotes After transfection, LC-1 ScFv expression was observed by Western blot analysis and the expression of cognate antigens was down-regulated as shown in ELISA assay.
T7 653-814 Sentence denotes After transfection, LC-1 ScFv expression was observed by Western blot analysis and the expression of cognate antigens was down-regulated as shown in ELISA assay.
TextSentencer_T8 815-947 Sentence denotes SPC-A-1-pDisplay-ScFv cells grew in vitro at lower speed than the control intact cells and the cells transfected with vacant vector.
T8 815-947 Sentence denotes SPC-A-1-pDisplay-ScFv cells grew in vitro at lower speed than the control intact cells and the cells transfected with vacant vector.
TextSentencer_T9 948-1132 Sentence denotes Flow cytometry analysis detected a substantial increase in G1 phase and decrease in S phase in population of SPC-A-1-pDisplay-ScFv cells compared to SPC-A-1 and SPC-A-1-pDisplay cells.
T9 948-1132 Sentence denotes Flow cytometry analysis detected a substantial increase in G1 phase and decrease in S phase in population of SPC-A-1-pDisplay-ScFv cells compared to SPC-A-1 and SPC-A-1-pDisplay cells.
TextSentencer_T10 1133-1246 Sentence denotes Semi-quantitative RT-PCR analysis showed that c-myc expression was down-regulated in SPC-A-1-pDisplay-ScFv cells.
T10 1133-1246 Sentence denotes Semi-quantitative RT-PCR analysis showed that c-myc expression was down-regulated in SPC-A-1-pDisplay-ScFv cells.
TextSentencer_T11 1247-1504 Sentence denotes It seems that the antigens recognized by LC-1 may be in some way involved in a growth stimulating pathway and the antibody blocking of the function of the antigens shut down the pathway and thus down-regulate the expression of c-myc and growth of the cells.
T11 1247-1504 Sentence denotes It seems that the antigens recognized by LC-1 may be in some way involved in a growth stimulating pathway and the antibody blocking of the function of the antigens shut down the pathway and thus down-regulate the expression of c-myc and growth of the cells.